Cloned (Comment) | Organism |
---|---|
expression of the the cyano-epsilon, CF1-epsilon and the chimeric epsilon subunits in Escherichia coli strain BL21(DE3) as soluble proteins | Thermosynechococcus vestitus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | inhibition of ATPase activity by the cyanobacterial epsilon subunit and the chimeric subunits composed of the N-terminal domain from the cyanobacterium and the C-terminal domain from spinach, overview | Thermosynechococcus vestitus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | ATP synthase contains an intrinsic inhibitor subunit epsilon, that acts as an endogenous inhibitor of chloroplast F1-ATPase, structure-function analysis, overview. Inhibition of ATPase activity by the cyanobacterial epsilon subunit and the chimaeric subunits composed of the N-terminal domain from the cyanobacterium and the C-terminal domain from spinach, overview | Thermosynechococcus vestitus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast membrane | - |
Thermosynechococcus vestitus | 31969 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + H+/out | Thermosynechococcus vestitus | the enzyme cannot synthesize ATP in the dark, but may catalyze futile ATP hydrolysis reactions | ATP + H2O + H+/in | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
strain BP-1 | - |
Renatured (Comment) | Organism |
---|---|
denaturation of recombinant the cyano-epsilon, CF1-epsilon and the chimeric epsilon subunits, expressed from Escherichia coli strain BL21(DE3) as soluble proteins, using 8 M urea, and subsequent refolding | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + H+/out | the enzyme cannot synthesize ATP in the dark, but may catalyze futile ATP hydrolysis reactions | Thermosynechococcus vestitus | ATP + H2O + H+/in | - |
r | |
additional information | structure-function relationship of the intrinsic inhibitor subunit epsilon subunit in F1 from photosynthetic organism, overview | Thermosynechococcus vestitus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure-function relationship of the intrinsic inhibitor subunit epsilon subunit in F1 from photosynthetic organism, NMR structure of the epsilon subunits, wild-type and mrecombinant/chimeric, overview. Analysis of the flexibility of the C-terminal domains using molecular dynamics simulations, overview | Thermosynechococcus vestitus |
Synonyms | Comment | Organism |
---|---|---|
ATP synthase | - |
Thermosynechococcus vestitus |
F1-ATPase | - |
Thermosynechococcus vestitus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | rate constants of the epsilon-binding obtained from epsilon-inhibition, overview | Thermosynechococcus vestitus |