Literature summary for 7.1.2.2 extracted from

Mueller, D.M.; Puri, N.; Kabaleeswaran, V.; Terry, C.; Leslie, A.G.; Walker, J.E.
Ni-chelate-affinity purification and crystallization of the yeast mitochondrial F1-ATPase (2004), Protein Expr. Purif., 37, 479-485.

Search for more literature for 7.1.2.2

Application

Application	Comment	Organism
additional information	simple and inexpensive method to grow yeast to high density and purify the mitochondrial F1-ATPase quickly and efficiently	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
ATPase genetically modified to include a His6 Ni affinity tag on the amino end of the mature beta-subunit, imported into mitochondrion, expression of the the purified chimeric enzyme in Escherichia coli DMY301	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
by sitting drop method at 23°C, to better than 2.8 A resolution	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Saccharomyces cerevisiae	5739	-

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae DK8	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni-chelate affinity chromatography and gel filtration	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
4°C, overnight	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + H+/in	-	Saccharomyces cerevisiae	ADP + phosphate + H+/out	-	r
ATP + H2O + H+/in	-	Saccharomyces cerevisiae DK8	ADP + phosphate + H+/out	-	r

Subunits

Subunits	Comment	Organism
More	subunits alpha, beta, gamma, delta and sigma of the purified chimeric enzyme, with the stoichiometry of 3:3:1:1:1, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
F1-ATPase	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)