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Literature summary for 7.1.1.9 extracted from

  • Bisson, R.; Schiavo, G.; Papini, E.
    Cytochrome c oxidase from the slime mold Dictyostelium discoideum: purification and characterization (1985), Biochemistry, 24, 7845-7852.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00126
-
ferrocytochrome c 2 Km values for horse heart, Neurospora crassa, Saccharomyces cerevisiea and Candida krusei cytochrome c Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5700
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
11000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
13000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
19000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
29500
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
55000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic chromatography, affinity chromatography on horse cytochrome c Dictyostelium discoideum

Storage Stability

Storage Stability Organism
-80°C, 5 mM Tris-cacodylate buffer, pH 7.6 Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + O2 + H+
-
Dictyostelium discoideum ferricytochrome c + H2O
-
r

Subunits

Subunits Comment Organism
? x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
158
-
ferrocytochrome c horse cytochrome c Dictyostelium discoideum
300
-
ferrocytochrome c
-
Dictyostelium discoideum