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Literature summary for 7.1.1.8 extracted from

  • Gutierrez-Cirlos, E.B.; Trumpower, B.L.
    Inhibitory analogs of ubiquinol act anti-cooperatively on the yeast cytochrome bc1 complex. Evidence for an alternating, half-of-the-sites mechanism of ubiquinol oxidation (2002), J. Biol. Chem., 277, 1195-1202.
    View publication on PubMed
Search for more literature for 7.1.1.8

Activating Compound

Activating Compound Comment Organism Structure
sucrose required for full in vitro activity, at 250 mM Saccharomyces cerevisiae
Tween 20 required for full in vitro activity, at 0.01% Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Antimycin
-
 Saccharomyces cerevisiae
methoxyacrylate stilbene inhibitory analogue of ubiquinol act anti-cooperatively on the enzyme, inhibitor blocks the enzymes center P with a stoichiometry of 0.5 per enzyme molecule Saccharomyces cerevisiae
additional information inhibition and inhibitor binding mechanisms Saccharomyces cerevisiae
Myxothiazol inhibitory analogue of ubiquinol act anti-cooperatively on the enzyme, inhibitor blocks the enzymes center P with a stoichiometry of 0.5 per enzyme molecule Saccharomyces cerevisiae
Stigmatellin inhibitory analogue of ubiquinol act anti-cooperatively on the enzyme, inhibitor blocks the enzymes center P with a stoichiometry of 0.5 per enzyme molecule Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pre-steady-state kinetics Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+[side 2] alternating half-of-the-sites mechanism of ubiquinol oxidation, enzyme links electron transfer from ubiquinol to cytochrome c by a protonmotive Q cycle mechanism in which ubiquinol is oxidized at one center in the enzyme, referred to as center P, und ubiquinone is re-reduced at a second center, referred to as center N Saccharomyces cerevisiae
a

Storage Stability

Storage Stability Organism
4°C, 0.003 mM purified enzyme, 0.01% Tween 20, 250 mM sucrose, 1 week, stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decylubiquinol + 2 ferricytochrome c
-
 Saccharomyces cerevisiae decylubiquinone + 2 ferrocytochrome c + H+ [side 2]
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
cytochrome bc1 complex
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 room temperature, assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
140 240 decylubiquinol different purified enzyme preparations, in 0.01% Tween 20, 250 mM sucrose Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000025
-
 methoxyacrylate stilbene pH 7.0, 22°C Saccharomyces cerevisiae