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Literature summary for 7.1.1.8 extracted from
- Isolation of the eleven protein subunits of the bc1 complex from beef heart (1986), Methods Enzymol., 126, 224-237.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
| mitochondrion | - |
Neurospora crassa | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 6400 | - |
- |
Bos taurus |
| 7200 | - |
- |
Bos taurus |
| 8000 | - |
DCCD-binding protein, SDS-PAGE | Bos taurus |
| 9200 | - |
hinge protein, SDS-PAGE | Bos taurus |
| 9500 | - |
- |
Bos taurus |
| 13400 | - |
Q-binding protein, SDS-PAGE | Bos taurus |
| 25000 | - |
iron-sulfur protein [Fe2-S2], SDS-PAGE | Saccharomyces cerevisiae |
| 25000 | - |
iron-sulfur protein [Fe2-S2], SDS-PAGE | Bos taurus |
| 25000 | - |
iron-sulfur protein [Fe2-S2], SDS-PAGE | Neurospora crassa |
| 27900 | - |
cytochrome c1, SDS-PAGE | Bos taurus |
| 43700 | - |
cytochrome b, SDS-PAGE | Bos taurus |
| 44000 | - |
- |
Saccharomyces cerevisiae |
| 44000 | - |
c1 subcomplex, SDS-PAGE | Bos taurus |
| 47000 | - |
core II, SDS-PAGE | Bos taurus |
| 49000 | - |
core I, SDS-PAGE | Bos taurus |
| 216000 | - |
bc1 subcomplex, SDS-PAGE | Bos taurus |
| 248000 | - |
bc1 complex, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Neurospora crassa | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, isolated complex III is stored in presence of 50% glycerol, can be used for functional studies up to a month, for preparation of subunits only freshly prepared bc1 complex, because storage leads to partial degradation of some subunits by proteases | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ubiquinol + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? |  |
| ubiquinol + 2 ferricytochrome c | - |
Bos taurus | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Neurospora crassa | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| undecamer | - |
Bos taurus |
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Release 2023.1 (January 2023)
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