Any feedback?
Literature summary for 7.1.1.8 extracted from
- Characterization of purified cytochrome b-c1 complex from Rhodopseudomonas sphaeroides R-26 (1984), J. Biol. Chem., 259, 5752-5760.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole | - |
Bos taurus |  |
| 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole | - |
Cereibacter sphaeroides | |
| antimycin A | - |
Bos taurus | |
| antimycin A | - |
Cereibacter sphaeroides | |
| antimycin A | - |
Spinacia oleracea | |
| additional information | insensitive to N',N'-dicyclohexylcarbodiimide | Cereibacter sphaeroides | |
| Phospholipase A2 | - |
Cereibacter sphaeroides | |
| undecyl-hydroxy-dioxobenzoxythiazole | - |
Bos taurus | |
| undecyl-hydroxy-dioxobenzoxythiazole | - |
Cereibacter sphaeroides | |
| undecyl-hydroxy-dioxobenzoxythiazole | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
ubiquinol-10 | - |
Cereibacter sphaeroides | |
| 0.006 | - |
ubiquinol-10 | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chromatophore | - |
Cereibacter sphaeroides | - |
- |
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron-sulfur cluster | - |
Bos taurus | |
| Iron-sulfur cluster | - |
Cereibacter sphaeroides | |
| Iron-sulfur cluster | - |
Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
cytochrome c, SDS-PAGE | Cereibacter sphaeroides |
| 48000 | - |
cytochrome b, SDS-PAGE | Cereibacter sphaeroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ubiquinone-10 + ferricytochrome c | Bos taurus | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Cereibacter sphaeroides | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Spinacia oleracea | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Cereibacter sphaeroides GA | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | Cereibacter sphaeroides R-26 | - |
ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Cereibacter sphaeroides | - |
- |
- |
| Cereibacter sphaeroides | - |
formerly Rhodopseudomonas sphaeroides | - |
| Cereibacter sphaeroides | - |
blue-green mutant | - |
| Cereibacter sphaeroides GA | - |
- |
- |
| Cereibacter sphaeroides R-26 | - |
blue-green mutant | - |
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cereibacter sphaeroides |
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for several weeks | Cereibacter sphaeroides |
| -80°C, lyophilized in 50% v/v glycerol | Cereibacter sphaeroides |
| 0°C, stable for several hours | Cereibacter sphaeroides |
| 20°C loses 13% of its activity after 1 h | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1 | - |
Cereibacter sphaeroides | 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+[side 2] | - |
? | |
| 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1 | - |
Cereibacter sphaeroides GA | 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+[side 2] | - |
? | |
| 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1 | - |
Cereibacter sphaeroides R-26 | 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+[side 2] | - |
? | |
| 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1 | - |
Cereibacter sphaeroides | 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+ | - |
? | |
| 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1 | - |
Cereibacter sphaeroides GA | 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+ | - |
? | |
| 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1 | - |
Cereibacter sphaeroides R-26 | 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Bos taurus | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Cereibacter sphaeroides | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Spinacia oleracea | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Cereibacter sphaeroides GA | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol + 2 ferricytochrome c | - |
Cereibacter sphaeroides R-26 | ubiquinone + 2 ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinol 10 + cytochrome c | - |
Bos taurus | ubiquinone 10 + reduced cytochrome c | - |
? | |
| ubiquinol 10 + cytochrome c | - |
Cereibacter sphaeroides | ubiquinone 10 + reduced cytochrome c | - |
? | |
| ubiquinol 10 + cytochrome c | - |
Cereibacter sphaeroides GA | ubiquinone 10 + reduced cytochrome c | - |
? | |
| ubiquinol 10 + cytochrome c | - |
Cereibacter sphaeroides R-26 | ubiquinone 10 + reduced cytochrome c | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Bos taurus | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Cereibacter sphaeroides | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Spinacia oleracea | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Cereibacter sphaeroides GA | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
| ubiquinone-10 + ferricytochrome c | - |
Cereibacter sphaeroides R-26 | ubiquinol-10 + ferrocytochrome c + 2 H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| b6/f complex | - |
Spinacia oleracea |
| cytochrome b-c1 complex | - |
Cereibacter sphaeroides |
| cytochrome b-c2 complex | - |
Cereibacter sphaeroides |
| plastoquinol-plastocyanin oxidoreductase | - |
Spinacia oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
stable for several hours | Cereibacter sphaeroides |
| 37 | - |
stable at lower temperatures, becomes less stable when incubation temperature is raised, half-life time at 37°C is 15 min | Cereibacter sphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | - |
Cereibacter sphaeroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | irreversibly denatured at pH lower than 5.0, stable at neutral pH, at pH 9.5 40% of the enzyme is irreversibly denatured after 19h | Cereibacter sphaeroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b | - |
Bos taurus | |
| cytochrome b | - |
Cereibacter sphaeroides | |
| cytochrome c1 | - |
Bos taurus | |
| cytochrome c1 | - |
Cereibacter sphaeroides | |
| Phospholipid | - |
Bos taurus | |
| Phospholipid | - |
Cereibacter sphaeroides | |
| ubiquinone | - |
Bos taurus | |
| ubiquinone | - |
Cereibacter sphaeroides | |
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