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Literature summary for 7.1.1.4 extracted from

  • Gleissner, M.; Kaiser, U.; Antonopoulos, E.; Schäfer, G.
    The archaeal SoxABCD complex is a proton pump in Sulfolobus acidocaldarius (1997), J. Biol. Chem., 272, 8417-8426.
    View publication on PubMed
Search for more literature for 7.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
cyanide 2 mM, oxidation of caldariella quinol and N,N,N',N'-tetramethyl-1,4-phenylenediamine is completely inhibited Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 caldariellaquinol pH 6.5, 50°C, enzyme in liposomes (tetraether lipids) Sulfolobus acidocaldarius
0.036
-
 caldariellaquinol pH 6.5, 50°C, enzyme in detergent micelle (dodecyl-beta-D-maltoside) Sulfolobus acidocaldarius
0.046
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 6.5, 40°C, enzyme in liposomes (tetraether lipids) Sulfolobus acidocaldarius
0.067
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 6.5, 40°C, enzyme in detergent micelle (dodecyl-beta-D-maltoside) Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
copper the enzyme contains one copper atom Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
 gel filtration Sulfolobus acidocaldarius
570000
-
 when membrane solubilization is carried out at low salt concentration, an oligomeric state of the oxidase is obtained Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius P39480 and P98004 and P39479 and P39477 P39480 (soxC) and P98004 (soxB) and P39479 (soxA) and P39477 (Saci_2086)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caldariellaquinol + O2 + n H+/in the SoxABCD complex is a proton-pumping quinol oxidase. With N,N,N',N'-tetramethyl-1,4-phenylenediamine as a reductant, the SoxABCD complex reconstituted into liposomes generates a proton motive force. The purified SoxABCD oxidase does not react with cytochrome c or blue copper proteins such as halocyanine or azurine as electron donors. However, considerable turnover numbers are found with N,N,N',N'-tetramethyl-1,4-phenylenediamine or caldariella quinol, confirming that in vivo the enzyme is acting as a quinol oxidase Sulfolobus acidocaldarius caldariellaquinone + H2O + n H+/out
-
 ?
N,N,N',N'-tetramethyl-1,4-phenylenediamine + O2 + n H+/in the SoxABCD complex is a proton-pumping quinol oxidase. With N,N,N',N'-tetramethyl-1,4-phenylenediamine as a reductant, the SoxABCD complex reconstituted into liposomes generates a proton motive force. The purified SoxABCD oxidase does not react with cytochrome c or blue copper proteins such as halocyanine or azurin as electron donors. However, considerable turnover numbers are found with N,N,N',N'-tetramethyl-1,4-phenylenediamine or caldariellaquinol, confirming that in vivo the enzyme is acting as a quinol oxidase Sulfolobus acidocaldarius ?
-
 ?

Synonyms

Synonyms Comment Organism
SoxABCD complex
-
 Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
157
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 6.5, 40°C, enzyme in detergent micelle (dodecyl-beta-D-maltoside) Sulfolobus acidocaldarius
239
-
 caldariellaquinol pH 6.5, 50°C, enzyme in liposomes (tetraether lipids) Sulfolobus acidocaldarius
393
-
 caldariellaquinol pH 6.5, 50°C, enzyme in detergent micelle (dodecyl-beta-D-maltoside) Sulfolobus acidocaldarius
404
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 6.5, 40°C, enzyme in liposomes (tetraether lipids) Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains four heme a redox centers. The EPR spectra indicate the presence of three low spin and one high spin heme species, the latter associated with the binuclear heme CuB site. The standard midpoint potentials of the cytochrome a587 heme centers are +210 and +270 mV, respectively Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2300
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 6.5, 40°C, enzyme in detergent micelle (dodecyl-beta-D-maltoside) Sulfolobus acidocaldarius
8700
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine pH 6.5, 40°C, enzyme in liposomes (tetraether lipids) Sulfolobus acidocaldarius
9200
-
 caldariellaquinol pH 6.5, 50°C, enzyme in liposomes (tetraether lipids) Sulfolobus acidocaldarius
11000
-
 caldariellaquinol pH 6.5, 50°C, enzyme in detergent micelle (dodecyl-beta-D-maltoside) Sulfolobus acidocaldarius