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Literature summary for 7.1.1.3 extracted from

  • Choi, S.K.; Lin, M.T.; Ouyang, H.; Gennis, R.B.
    Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli (2017), Biochim. Biophys. Acta, 1858, 366-370 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43 BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D36V the mutant of subunit III shows 160% activity compared to the wild type enzyme Escherichia coli
E164A the mutant of subunit I shows 28% activity compared to the wild type enzyme Escherichia coli
E164K the mutant of subunit I shows 54% activity compared to the wild type enzyme Escherichia coli
E259A the mutant of subunit II shows 135% activity compared to the wild type enzyme Escherichia coli
E259K the mutant of subunit II shows 97% activity compared to the wild type enzyme Escherichia coli
F165A the mutant of subunit I shows 37% activity compared to the wild type enzyme Escherichia coli
F29I the mutant of subunit III shows 115% activity compared to the wild type enzyme Escherichia coli
K25L the mutant of subunit III shows75 % activity compared to the wild type enzyme Escherichia coli
L171A the mutant of subunit I shows 79% activity compared to the wild type enzyme Escherichia coli
Q167A the mutant of subunit I shows 57% activity compared to the wild type enzyme Escherichia coli
Q167K the mutant of subunit I shows 75% activity compared to the wild type enzyme Escherichia coli
Q195L the mutant of subunit I shows 119% activity compared to the wild type enzyme Escherichia coli
R176A the mutant of subunit III shows 70% activity compared to the wild type enzyme Escherichia coli
S177A the mutant of subunit I shows 91% activity compared to the wild type enzyme Escherichia coli
T168A the mutant of subunit I shows 118% activity compared to the wild type enzyme Escherichia coli
T247V the mutant of subunit I shows 173% activity compared to the wild type enzyme Escherichia coli
W136A the mutant of subunit II shows 89% activity compared to the wild type enzyme Escherichia coli
W136K the mutant of subunit II shows 105% activity compared to the wild type enzyme Escherichia coli
W156A the mutant of subunit III shows 70% activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
ubiquinol-1 mutant enzyme D36V, at pH 7.0 and 25°C Escherichia coli
0.02
-
ubiquinol-1 mutant enzyme E164A, at pH 7.0 and 25°C Escherichia coli
0.021
-
ubiquinol-1 mutant enzyme T247V, at pH 7.0 and 25°C Escherichia coli
0.026
-
ubiquinol-1 mutant enzyme Q167K, at pH 7.0 and 25°C Escherichia coli
0.027
-
ubiquinol-1 mutant enzyme E259A, at pH 7.0 and 25°C Escherichia coli
0.027
-
ubiquinol-1 mutant enzyme E259K, at pH 7.0 and 25°C Escherichia coli
0.027
-
ubiquinol-1 mutant enzyme Q167A, at pH 7.0 and 25°C Escherichia coli
0.031
-
ubiquinol-1 mutant enzyme T168A, at pH 7.0 and 25°C Escherichia coli
0.032
-
ubiquinol-1 mutant enzyme S177A, at pH 7.0 and 25°C Escherichia coli
0.033
-
ubiquinol-1 mutant enzyme F165A, at pH 7.0 and 25°C Escherichia coli
0.033
-
ubiquinol-1 mutant enzyme W136A, at pH 7.0 and 25°C Escherichia coli
0.039
-
ubiquinol-1 mutant enzyme W136K, at pH 7.0 and 25°C Escherichia coli
0.042
-
ubiquinol-1 mutant enzyme L171A, at pH 7.0 and 25°C Escherichia coli
0.046
-
ubiquinol-1 mutant enzyme E164K, at pH 7.0 and 25°C Escherichia coli
0.05
-
ubiquinol-1 wild type enzyme, at pH 7.0 and 25°C Escherichia coli
0.067
-
ubiquinol-1 mutant enzyme Q195L, at pH 7.0 and 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ contains copper Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquinol-1 + O2 + H+[side 1]
-
Escherichia coli ubiquinone-1 + H2O + H+[side 2]
-
?

Synonyms

Synonyms Comment Organism
cytochrome bo3 oxidase
-
Escherichia coli
cytochrome bo3 ubiquinol oxidase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
heme
-
Escherichia coli