Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43 BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D36V | the mutant of subunit III shows 160% activity compared to the wild type enzyme | Escherichia coli |
E164A | the mutant of subunit I shows 28% activity compared to the wild type enzyme | Escherichia coli |
E164K | the mutant of subunit I shows 54% activity compared to the wild type enzyme | Escherichia coli |
E259A | the mutant of subunit II shows 135% activity compared to the wild type enzyme | Escherichia coli |
E259K | the mutant of subunit II shows 97% activity compared to the wild type enzyme | Escherichia coli |
F165A | the mutant of subunit I shows 37% activity compared to the wild type enzyme | Escherichia coli |
F29I | the mutant of subunit III shows 115% activity compared to the wild type enzyme | Escherichia coli |
K25L | the mutant of subunit III shows75 % activity compared to the wild type enzyme | Escherichia coli |
L171A | the mutant of subunit I shows 79% activity compared to the wild type enzyme | Escherichia coli |
Q167A | the mutant of subunit I shows 57% activity compared to the wild type enzyme | Escherichia coli |
Q167K | the mutant of subunit I shows 75% activity compared to the wild type enzyme | Escherichia coli |
Q195L | the mutant of subunit I shows 119% activity compared to the wild type enzyme | Escherichia coli |
R176A | the mutant of subunit III shows 70% activity compared to the wild type enzyme | Escherichia coli |
S177A | the mutant of subunit I shows 91% activity compared to the wild type enzyme | Escherichia coli |
T168A | the mutant of subunit I shows 118% activity compared to the wild type enzyme | Escherichia coli |
T247V | the mutant of subunit I shows 173% activity compared to the wild type enzyme | Escherichia coli |
W136A | the mutant of subunit II shows 89% activity compared to the wild type enzyme | Escherichia coli |
W136K | the mutant of subunit II shows 105% activity compared to the wild type enzyme | Escherichia coli |
W156A | the mutant of subunit III shows 70% activity compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
ubiquinol-1 | mutant enzyme D36V, at pH 7.0 and 25°C | Escherichia coli | |
0.02 | - |
ubiquinol-1 | mutant enzyme E164A, at pH 7.0 and 25°C | Escherichia coli | |
0.021 | - |
ubiquinol-1 | mutant enzyme T247V, at pH 7.0 and 25°C | Escherichia coli | |
0.026 | - |
ubiquinol-1 | mutant enzyme Q167K, at pH 7.0 and 25°C | Escherichia coli | |
0.027 | - |
ubiquinol-1 | mutant enzyme E259A, at pH 7.0 and 25°C | Escherichia coli | |
0.027 | - |
ubiquinol-1 | mutant enzyme E259K, at pH 7.0 and 25°C | Escherichia coli | |
0.027 | - |
ubiquinol-1 | mutant enzyme Q167A, at pH 7.0 and 25°C | Escherichia coli | |
0.031 | - |
ubiquinol-1 | mutant enzyme T168A, at pH 7.0 and 25°C | Escherichia coli | |
0.032 | - |
ubiquinol-1 | mutant enzyme S177A, at pH 7.0 and 25°C | Escherichia coli | |
0.033 | - |
ubiquinol-1 | mutant enzyme F165A, at pH 7.0 and 25°C | Escherichia coli | |
0.033 | - |
ubiquinol-1 | mutant enzyme W136A, at pH 7.0 and 25°C | Escherichia coli | |
0.039 | - |
ubiquinol-1 | mutant enzyme W136K, at pH 7.0 and 25°C | Escherichia coli | |
0.042 | - |
ubiquinol-1 | mutant enzyme L171A, at pH 7.0 and 25°C | Escherichia coli | |
0.046 | - |
ubiquinol-1 | mutant enzyme E164K, at pH 7.0 and 25°C | Escherichia coli | |
0.05 | - |
ubiquinol-1 | wild type enzyme, at pH 7.0 and 25°C | Escherichia coli | |
0.067 | - |
ubiquinol-1 | mutant enzyme Q195L, at pH 7.0 and 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | contains copper | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol-1 + O2 + H+[side 1] | - |
Escherichia coli | ubiquinone-1 + H2O + H+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome bo3 oxidase | - |
Escherichia coli |
cytochrome bo3 ubiquinol oxidase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli |