Cloned (Comment) | Organism |
---|---|
recombinant expression of Cyt-bo3 samples in Escherichia coli strain C43, each plasmid encodes the cyoABCDE operon expressing Cyt-bo3 with a 6-His tag on the C-terminus of subunit II. Functional cell-free in vitro expression of Cyt-bo3, employing T7 promoter, and insertion into the artificial membrane | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged on subunit II Cyt-bo3 from Escherichia coli strain C43 by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzymatic activity of Cyt-bo3 was estimated by measuring consumption of oxygen for ubiquinol oxidation | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cyt-bo3 | - |
Escherichia coli |
cytochrome bo3 ubiquinol oxidase | - |
Escherichia coli |