Protein Variants | Comment | Organism |
---|---|---|
D75E | 135% activity compared to the wild type enzyme | Escherichia coli |
D75H | 4% activity compared to the wild type enzyme | Escherichia coli |
D75N | inactive | Escherichia coli |
D75R | inactive | Escherichia coli |
H98N | 1% activity compared to the wild type enzyme | Escherichia coli |
H98S | 2% activity compared to the wild type enzyme | Escherichia coli |
H98T | 1% activity compared to the wild type enzyme | Escherichia coli |
Q101N | 5% activity compared to the wild type enzyme | Escherichia coli |
R71D | inactive | Escherichia coli |
R71D/D75R | inactive | Escherichia coli |
R71K | inactive | Escherichia coli |
R71Q | inactive | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-heptyl-4-hydroxyquinoline N-oxide | - |
Escherichia coli | |
aurachin C1-10 | competitive inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
ubiquinol-1 | mutant enzyme D188N, at 37°C, pH 7.0 | Escherichia coli | |
0.053 | - |
ubiquinol-1 | wild type enzyme, at 37°C, pH 7.0 | Escherichia coli | |
0.056 | - |
ubiquinol-1 | mutant enzyme D188A, at 37°C, pH 7.0 | Escherichia coli | |
0.06 | - |
ubiquinol-1 | mutant enzyme R257Q, at 37°C, pH 7.0 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | copper-containing enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol-1 + O2 + H+/in | - |
Escherichia coli | ubiquinone-1 + H2O + H+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome bo3 ubiquinol oxidase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme b and heme o3 | Escherichia coli | |
ubiquinone-8 | the enzyme contains one equivalent of ubiquinone-8 | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000012 | - |
aurachin C1-10 | mutant enzyme R257Q, at 37°C, pH 7.0 | Escherichia coli | |
0.000015 | - |
aurachin C1-10 | wild type enzyme, at 37°C, pH 7.0 | Escherichia coli | |
0.000025 | - |
aurachin C1-10 | mutant enzyme D188A, at 37°C, pH 7.0 | Escherichia coli | |
0.00074 | - |
2-heptyl-4-hydroxyquinoline N-oxide | wild type enzyme, at 37°C, pH 7.0 | Escherichia coli |