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Literature summary for 7.1.1.3 extracted from

  • Yap, L.L.; Lin, M.T.; Ouyang, H.; Samoilova, R.I.; Dikanov, S.A.; Gennis, R.B.
    The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli (2010), Biochim. Biophys. Acta, 1797, 1924-1932.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D75E 135% activity compared to the wild type enzyme Escherichia coli
D75H 4% activity compared to the wild type enzyme Escherichia coli
D75N inactive Escherichia coli
D75R inactive Escherichia coli
H98N 1% activity compared to the wild type enzyme Escherichia coli
H98S 2% activity compared to the wild type enzyme Escherichia coli
H98T 1% activity compared to the wild type enzyme Escherichia coli
Q101N 5% activity compared to the wild type enzyme Escherichia coli
R71D inactive Escherichia coli
R71D/D75R inactive Escherichia coli
R71K inactive Escherichia coli
R71Q inactive Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-heptyl-4-hydroxyquinoline N-oxide
-
Escherichia coli
aurachin C1-10 competitive inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
ubiquinol-1 mutant enzyme D188N, at 37°C, pH 7.0 Escherichia coli
0.053
-
ubiquinol-1 wild type enzyme, at 37°C, pH 7.0 Escherichia coli
0.056
-
ubiquinol-1 mutant enzyme D188A, at 37°C, pH 7.0 Escherichia coli
0.06
-
ubiquinol-1 mutant enzyme R257Q, at 37°C, pH 7.0 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu copper-containing enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquinol-1 + O2 + H+/in
-
Escherichia coli ubiquinone-1 + H2O + H+/out
-
?

Synonyms

Synonyms Comment Organism
cytochrome bo3 ubiquinol oxidase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
heme heme b and heme o3 Escherichia coli
ubiquinone-8 the enzyme contains one equivalent of ubiquinone-8 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000012
-
aurachin C1-10 mutant enzyme R257Q, at 37°C, pH 7.0 Escherichia coli
0.000015
-
aurachin C1-10 wild type enzyme, at 37°C, pH 7.0 Escherichia coli
0.000025
-
aurachin C1-10 mutant enzyme D188A, at 37°C, pH 7.0 Escherichia coli
0.00074
-
2-heptyl-4-hydroxyquinoline N-oxide wild type enzyme, at 37°C, pH 7.0 Escherichia coli