Protein Variants | Comment | Organism |
---|---|---|
D178N | mutant of subunit NuoL, about 80% of electron transfer activity of wild-type | Escherichia coli |
D303A | mutant of subunit NuoL, about 60% of electron transfer activity of wild-type | Escherichia coli |
D3400N | mutant of subunit NuoL, about 35% of electron transfer activity of wild-type | Escherichia coli |
D400A | mutant of subunit NuoL, about 80% of electron transfer activity of wild-type | Escherichia coli |
D400E | mutant of subunit NuoL, about 50% of electron transfer activity of wild-type | Escherichia coli |
E144Q | mutant of subunit NuoL, about 10% of electron transfer activity of wild-type | Escherichia coli |
H334Q | mutant of subunit NuoL, about 50% of electron transfer activity of wild-type | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-(N-ethyl-N-isopropyl)-amiloride | 0.015 mM, up to 40% decrease in the proton pumping activity of the wild-type and subunit NuoL mutants D303A, D400A, D400E, no significant change in mutant D178N | Escherichia coli | |
Zn2+ | at 2 mM Zn2+, proton pumping of the wild-type is selectively inhibited without affecting electron transfer, no inhibition in proton pumping of mutants D178N and D400A | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | almost no proton pumping activities detected in the absence of Mg2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
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General Information | Comment | Organism |
---|---|---|
physiological function | subunit NuoL is involved in the high efficiency pumping mechanism in complex I | Escherichia coli |