Cloned (Comment) | Organism |
---|---|
expression of recombinant enzyme subunits in Escherichia coli, quantitative expression analysis | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mrpA or mrpD deletion mutant strains, the deletion strains can be complemented in trans by their respective Mrp protein, but expression of MrpA in the Bacillus subtilis DELTAmrpD strain and vice versa does not improve growth at pH 7.4, mutant strains growth phenotypes, overview | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + ubiquinone + 6 H+[side 1] | Bacillus subtilis | - |
NAD+ + ubiquinol + 7 H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
genes mrpA or mrpD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + ubiquinone + 6 H+[side 1] | - |
Bacillus subtilis | NAD+ + ubiquinol + 7 H+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
complex I | - |
Bacillus subtilis |
NADH:ubiquinone oxidoreductase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | the complex I subunits NuoL, NuoM and NuoN are homologous to two proteins, MrpA and MrpD, from one particular class of Na+/H+ antiporters. In many bacteria MrpA and MrpD are encoded by an operon comprising 6-7 conserved genes | Bacillus subtilis |
malfunction | deletion of either mrpA or mrpD from the Bacillus subtilis chromosome resulted in a Na+ and pH sensitive growth phenotype. NuoL can rescue Bacillus subtilis DELTAmrpA, but improved the growth of Bacillus subtilis DELTAmrpD only slightly. NuoN can restore the wild type properties of Bacillus subtilis DELTAmrpD, but has no effect on the ?mrpA strain. Expression of NuoM does not result in any growth improvement under these conditions. This reveals that the complex I subunits NuoL, NuoM and NuoN also demonstrate functional specializations | Bacillus subtilis |
physiological function | MrpA transports Na+ whereas MrpD transports H+ in opposite directions, resulting in antiporter activity, besides one Na+ channel, NuoL, and two H+ channels, NuoM and NuoN, are present. | Bacillus subtilis |