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Literature summary for 7.1.1.2 extracted from

  • Bironaite, D.; Cenas, N.; Kulys, J.
    The rotenone-insensitive reduction of quinones and nitrocompounds by mitochondrial NADH:ubiquinone reductase (1991), Biochim. Biophys. Acta, 1060, 203-209.
    View publication on PubMed
Search for more literature for 7.1.1.2

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by ferricyanide above 1 mM Bos taurus
NAD+ NAD+ acts as a mixed-type inhibitor for both substrates in the NADH: ferricyanide reductase reaction of complex I Bos taurus
NADH NADH acts as a competitive inhibitor for ferricyanide, the reactions of with quinones and nitro compounds are not inhibited by NADH Bos taurus
Rhein i.e. 4,5-dioxy-anthraquinone-z-carbonic acid, competes for a NADH-binding site Bos taurus
rotenone 0.002 mM rotenone diminishes the bimolecular rate constant value for tetramethyl-1,4-benzoquinone and for 2,5-dimethyl-1,4-benzoquinone by 30 and 10%, respectively Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 5,8-dioxo-1,4-naphthoquinone pH 7.0, 25°C Bos taurus
0.09
-
 9,10-phenanthrenequinone pH 7.0, 25°C Bos taurus
0.12
-
 5-oxo-1,4-naphthoquinone pH 7.0, 25°C Bos taurus
0.4
-
 1,4-benzoquinone pH 7.0, 25°C Bos taurus
0.4
-
 2,5-dimethyl-1,4-benzoquinone pH 7.0, 25°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Bos taurus 2 ferrocyanide + NAD+ + H+
-
 ?
NADH + H+ + 1,4-benzoquinone
-
 Bos taurus NAD+ + 1,4-benzoquinol
-
 ?
NADH + H+ + 1,4-naphthoquinone
-
 Bos taurus NAD+ + 1,4-naphthoquinol
-
 ?
NADH + H+ + 2,5-dimethyl-1,4-benzoquinone
-
 Bos taurus NAD+ + 2,5-dimethyl-1,4-benzoquinol
-
 ?
NADH + H+ + 2-methyl-1,4-benzoquinone
-
 Bos taurus NAD+ + 2-methyl-1,4-benzoquinol
-
 ?
NADH + H+ + 2-methyl-1,4-naphthoquinone
-
 Bos taurus NAD+ + 2-methyl-1,4-naphthoquinol
-
 ?
NADH + H+ + 4-nitroacetophenone
-
 Bos taurus NAD+ + ?
-
 ?
NADH + H+ + 4-nitrobenzaldehyde
-
 Bos taurus NAD+ + ?
-
 ?
NADH + H+ + 4-nitrobenzoic acid
-
 Bos taurus NAD+ + ?
-
 ?
NADH + H+ + 5,8-dioxo-1,4-naphthoquinone
-
 Bos taurus NAD+ + 5,8-dioxo-1,4-naphthoquinol
-
 ?
NADH + H+ + 5-oxo-1,4-naphthoquinone
-
 Bos taurus NAD+ + 5-oxo-1,4-naphthoquinol
-
 ?
NADH + H+ + 9,10-phenanthrenequinone
-
 Bos taurus NAD+ + 9,10-phenanthrenequinol
-
 ?
NADH + H+ + adriamycin
-
 Bos taurus NAD+ + ?
-
 ?
NADH + H+ + nifuroxime
-
 Bos taurus NAD+ + ?
-
 ?
NADH + H+ + nitrobenzene
-
 Bos taurus NAD+ + ?
-
 ?
NADH + H+ + nitrofurantoin
-
 Bos taurus NAD+ + ?
-
 ?
NADH + H+ + tetramethyl-1,4-benzoquinone
-
 Bos taurus NAD+ + tetramethyl-1,4-benzoquinol
-
 ?
NADH + H+ + ubiquinone-1
-
 Bos taurus NAD+ + ubiquinol-1
-
 ?

Synonyms

Synonyms Comment Organism
complex I
-
 Bos taurus
NADH: ubiquinone reductase
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Bos taurus
iron-sulfur centre the enzyme contains 8-9 iron-sulfur clusters Bos taurus
NADH
-
 Bos taurus
ubiquinone
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
 NADH pH 7.0, 25°C Bos taurus