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Literature summary for 7.1.1.2 extracted from

  • Fisher, N.; Warman, A.J.; Ward, S.A.; Biagini, G.A.
    Type II NADH:quinone oxidoreductases of Plasmodium falciparum and Mycobacterium tuberculosis kinetic and high-throughput assays (2009), Methods Enzymol., 456, 303-320.
    View publication on PubMed
Search for more literature for 7.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

General Stability

General Stability Organism
not stable to repeated freeze-thaw cycles Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
1-hydroxy-2-octyl-4(1H)quinolone
-
 Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the membrane-bound respiratory enzymes differs from the canonical NADH: dehydrogenase (complex I), because it is not involved in the vectorial transfer of protons across membranes Mycobacterium tuberculosis 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + menaquinone Mycobacterium tuberculosis the enzyme plays an essential role in maintaining a reduced ubiquinone-pool during infection (Mycobacterium tuberculosis is the causative agents of tuberculosis). The enzyme is not only essential to parasite survival in vivo but may also contribute to the severity and outcome of disease. Type II NADH:quinone oxidoreductase the membrane-bound respiratory enzyme differs from the canonical NADH:dehydrogenase (complex I), because it is not involved in the vectorial transfer of protons across membranes. Mycobacterium tuberculosis contains a branched respiratory chain terminating in a cytochrome bd (quinol) oxidase and an aa3-type cytochrome c oxidase. Both chains are fed by a menaquinol (MQH2) pool that is generated by four dehydrogenases; one succinate menaquinone oxidoreductase (SQR), one multimeric type I NADH: dehydrogenase (complex I), and two type II NADH: menaquinone oxidoreductases (ndh and ndhA). Transposon insertion knockout strategy reveals that disruption of the ndh gene is lethal NAD+ + menaquinol
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 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Storage Stability

Storage Stability Organism
-80°C, stable for at least 6 months Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + decylubiquinone the enzyme is selective for NADH Mycobacterium tuberculosis NAD+ + decylubiquinol
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 ?
NADH + H+ + menaquinone the enzyme plays an essential role in maintaining a reduced ubiquinone-pool during infection (Mycobacterium tuberculosis is the causative agents of tuberculosis). The enzyme is not only essential to parasite survival in vivo but may also contribute to the severity and outcome of disease. Type II NADH:quinone oxidoreductase the membrane-bound respiratory enzyme differs from the canonical NADH:dehydrogenase (complex I), because it is not involved in the vectorial transfer of protons across membranes. Mycobacterium tuberculosis contains a branched respiratory chain terminating in a cytochrome bd (quinol) oxidase and an aa3-type cytochrome c oxidase. Both chains are fed by a menaquinol (MQH2) pool that is generated by four dehydrogenases; one succinate menaquinone oxidoreductase (SQR), one multimeric type I NADH: dehydrogenase (complex I), and two type II NADH: menaquinone oxidoreductases (ndh and ndhA). Transposon insertion knockout strategy reveals that disruption of the ndh gene is lethal Mycobacterium tuberculosis NAD+ + menaquinol
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 ?
NADH + H+ + ubiquinone-1
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 Mycobacterium tuberculosis NAD+ + ubiquinol-1
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 ?

Synonyms

Synonyms Comment Organism
NADH:ubiquinone oxidoreductase
-
 Mycobacterium tuberculosis
NDH
-
 Mycobacterium tuberculosis
Ndh/NdhA–type II NADH:(mena)quinone oxidoreductase
-
 Mycobacterium tuberculosis
type II NADH:quinone oxidoreductase
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH the enzyme is selective for NADH Mycobacterium tuberculosis