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Literature summary for 7.1.1.2 extracted from

  • Pohl, T.; Schneider, D.; Hielscher, R.; Stolpe, S.; Doerner, K.; Kohlstaedt, M.; Boettcher, B.; Hellwig, P.; Friedrich, T.
    Nucleotide-induced conformational changes in the Escherichia coli NADH:ubiquinone oxidoreductase (complex I) (2008), Biochem. Soc. Trans., 36, 971-975.
    View publication on PubMed
Search for more literature for 7.1.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADH
-
 Escherichia coli
1.8
-
 NADPH
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane electron microscopy reveals the two-part structure of the complex consisting of a peripheral and a membrane arm. The peripheral arm contains all known cofactors and the NADH-binding site, whereas the membrane arm has to be involved in proton translocation Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe enzyme contains Fe-S clusters Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + ubiquinone Escherichia coli the energy-converting NADH:ubiquinone oxidoreductase (respiratory complex I), couples the transfer of electrons from NADH to ubiquinone with the translocation of protons across the membrane NAD+ + ubiquinol
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + ubiquinone the energy-converting NADH:ubiquinone oxidoreductase (respiratory complex I), couples the transfer of electrons from NADH to ubiquinone with the translocation of protons across the membrane Escherichia coli NAD+ + ubiquinol
-
 ?
NADPH + H+ + ubiquinone
-
 Escherichia coli NADP+ + ubiquinol
-
 ?

Synonyms

Synonyms Comment Organism
NADH:ubiquinone oxidoreductase
-
 Escherichia coli
respiratory complex I
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH both arms of the Escherichia coli complex I are widened after the addition of NADH but not of NADPH. NADH-induced conformational changes are also detected in solution Escherichia coli
NADPH addition of NADH but not NADPH leads to an extension of the overall structure of the complex, although all cofactors of the complex are reduced by NADPH Escherichia coli