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Literature summary for 7.1.1.2 extracted from

  • Yadava, N.; Potluri, P.; Scheffler, I.E.
    Investigations of the potential effects of phosphorylation of the MWFE and ESSS subunits on complex I activity and assembly (2008), Int. J. Biochem. Cell Biol., 40, 447-460.
    View publication on PubMed
Search for more literature for 7.1.1.2

Application

Application Comment Organism
additional information if phosphorylation occurs in vivo, effects on complex I activity are significant Cricetulus griseus

Cloned(Commentary)

Cloned (Comment) Organism
constitutive expression of the tagged wild type, or mutant MWFE or ESSS proteins via a pTRIDENT-neo (tricistronic) vector with the EF1alpha promoter Cricetulus griseus

Protein Variants

Protein Variants Comment Organism
S2A mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus
S2A/S8A double mutant of phosphorylated subunit ESSS, mutant protein causes a complete failure to assemble Cricetulus griseus
S2E mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus
S30A mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus
S30E mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus
S55A mutant of phosphorylated subunit MWFE, functional complex I can be assembled, mutant protein is expressed at a lower level compared to the wild-type protein Cricetulus griseus
S55D mutant of phosphorylated subunit MWFE, assembly of complex I is completely blocked Cricetulus griseus
S55E mutant of phosphorylated subunit MWFE, assembly of complex I is completely blocked Cricetulus griseus
S55Q mutant of phosphorylated subunit MWFE, assembly of complex I is completely blocked Cricetulus griseus
S8A mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus
S8E mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus
T21A mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus
T21E mutant of phosphorylated subunit ESSS, shows lower levels of mature protein and a significantly reduced complex I activity Cricetulus griseus

Inhibitors

Inhibitors Comment Organism Structure
rotenone
-
 Cricetulus griseus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Cricetulus griseus 5739
-

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information CCL16-B2 cell and V79-G18 cell Cricetulus griseus
-

Synonyms

Synonyms Comment Organism
complex I
-
 Cricetulus griseus
NADH-ubiquinone oxidoreductase
-
 Cricetulus griseus