Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.1.2 extracted from

  • Flemming, D.; Hellwig, P.; Lepper, S.; Kloer, D.P.; Friedrich, T.
    Catalytic importance of acidic amino acids on subunit NuoB of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I) (2006), J. Biol. Chem., 281, 24781-24789.
    View publication on PubMed
Search for more literature for 7.1.1.2

Protein Variants

Protein Variants Comment Organism
D115N 45% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
D146N 59% of deamino-NADH oxidase activity of the wild type enzyme, one fourth reduced complex 1 content Escherichia coli
D152N 59% of deamino-NADH oxidase activity of the wild type enzyme, one third reduced complex 1 content Escherichia coli
D77E 54% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
D77N completely abolished electron transfer activity, 12% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
D94E 83% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
D94N completely abolished electron transfer activity, 12% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
E119Q 88% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
E163Q 76% of deamino-NADH oxidase activity of the wild type enzyme, one third reduced complex 1 content Escherichia coli
E67D 78% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli
E67Q completely abolished electron transfer activity, one fourth reduced complex 1 content, 10% of deamino-NADH oxidase activity of the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Piericidin A
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe-S contains 9 iron-sulfur clusters Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli ANN023
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Source 15Q column chromatography and Sephacryl S-300 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + deamino-NADH
-
 Escherichia coli 2 ferrocyanide + deamino-NAD+ + H+
-
 ?
2 ferricyanide + deamino-NADH
-
 Escherichia coli ANN023 2 ferrocyanide + deamino-NAD+ + H+
-
 ?
2 ferricyanide + NADH
-
 Escherichia coli 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricyanide + NADH
-
 Escherichia coli ANN023 2 ferrocyanide + NAD+ + H+
-
 ?
NADH + ubiquinone
-
 Escherichia coli NAD+ + ubiquinol
-
 ?
NADH + ubiquinone
-
 Escherichia coli ANN023 NAD+ + ubiquinol
-
 ?

Subunits

Subunits Comment Organism
tridecamer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
complex I
-
 Escherichia coli
NADH:ubiquinone oxidoreductase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Escherichia coli
NADH
-
 Escherichia coli