Any feedback?
Literature summary for 7.1.1.2 extracted from
- Steady-state kinetics and inhibitory action of antitubercular phenothiazines on Mycobacterium tuberculosis type-II NADH-menaquinone oxidoreductase (NDH-2) (2006), J. Biol. Chem., 281, 11456-11463.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Mycobacterium smegmatis | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dodecyl-beta-D-maltoside | leads to immediate loss of activity | Mycobacterium tuberculosis |  |
| Trifluoperazine | 0.05 mM | Mycobacterium tuberculosis | |
| Triton X-100 | leads to immediate loss of activity | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
calculated from sequence of cDNA | Mycobacterium tuberculosis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Triton X-100 | leads to immediate loss of activity | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultracentrifugation | Mycobacterium tuberculosis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, alkaline buffer with 40% (w/v) glycerol, one month, without loss of activity | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | does not react with NADPH or oxygen | Mycobacterium tuberculosis | ? | - |
? | |
| NADH + 2-methyl-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | NAD+ + 2-methyl-1,4-naphthoquinol | - |
? | |
| NADH + decylubiquinone | - |
Mycobacterium tuberculosis | NAD+ + decylubiquinol | - |
? | |
| NADH + H+ + 1,4-naphthoquinone | - |
Mycobacterium tuberculosis | NAD+ + 1,4-naphthoquinol | - |
? | |
| NADH + menaquinone | - |
Mycobacterium tuberculosis | NAD+ + menaquinol | - |
? | |
| NADH + phylloquinone | - |
Mycobacterium tuberculosis | NAD+ + phylloquinol | - |
? | |
| NADH + ubiquinone-1 | - |
Mycobacterium tuberculosis | NAD+ + ubiquinol-1 | - |
? | |
| NADH + ubiquinone-2 | - |
Mycobacterium tuberculosis | NAD+ + ubiquinol-2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDH-2 | - |
Mycobacterium tuberculosis |
| type-II NADH-menaquinone oxidoreductase | - |
Mycobacterium tuberculosis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
NDH-2 activity increases rapidly at pH 8.0 or above | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Mycobacterium tuberculosis | |
| NADH | - |
Mycobacterium tuberculosis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
