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Literature summary for 7.1.1.2 extracted from

  • Droese, S.; Galkin, A.; Brandt, U.
    Proton pumping by complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica reconstituted into proteoliposomes (2005), Biochim. Biophys. Acta, 1710, 87-95.
    View publication on PubMed
Search for more literature for 7.1.1.2

Inhibitors

Inhibitors Comment Organism Structure
9-amino-6-chloro-2-methoxyacridine 0.0005 mM Yarrowia lipolytica
carbonyl-cyanide-p-trifluoro-methoxy-phenylhydrazone 0.001 mM Yarrowia lipolytica
nigericin 0.0125 mM Yarrowia lipolytica
rotenone 0.001 mM Yarrowia lipolytica

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TSKgel 4000SW gel filtration Yarrowia lipolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
 in 20 mM MOPS containing 50 mM KCl at pH 7.4, using n-decylubiquinone as substrate Yarrowia lipolytica
4.8
-
 in 20 mM MOPS containing 50 mM KCl at pH 7.4, using ubiquinone-1 as substrate Yarrowia lipolytica
39.1
-
 in 20 mM MOPS containing 50 mM KCl at pH 7.4, using hexamineruthenium(III)-chloride as substrate Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + hexamineruthenium(III)-chloride
-
 Yarrowia lipolytica NAD+ + ?
-
 ?
NADH + n-decylubiquinone + H+
-
 Yarrowia lipolytica NAD+ + n-decylubiquinol
-
 ?
NADH + ubiquinone-1
-
 Yarrowia lipolytica NAD+ + ubiquinol-1
-
 ?

Synonyms

Synonyms Comment Organism
complex I
-
 Yarrowia lipolytica
NADH:ubiquinone oxidoreductase
-
 Yarrowia lipolytica

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Yarrowia lipolytica