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Literature summary for 7.1.1.2 extracted from

  • Sinegina, L.; Wikstroem, M.; Verkhovsky, M.I.; Verkhovskaya, M.L.
    Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters (2005), Biochemistry, 44, 8500-8506.
    View publication on PubMed
Search for more literature for 7.1.1.2

Activating Compound

Activating Compound Comment Organism Structure
asolectin
-
 Escherichia coli
dodecyl maltoside strong stimulation of activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
rolliniastatin strong inhibitor Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
[4Fe-4S] center
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MWC215
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Trisacryl M anion exchange column chromatography, Source 15Q column chromatography and ultracentrifugation Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
 membrane extract, using hexaammineruthenium-III-chloride as substrate Escherichia coli
90
-
 after purification, using hexaammineruthenium-III-chloride as substrate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + decylubiquinone
-
 Escherichia coli NAD+ + decylubiquinol
-
 ?
NADH + decylubiquinone
-
 Escherichia coli MWC215 NAD+ + decylubiquinol
-
 ?
NADH + hexaammineruthenium-III-chloride
-
 Escherichia coli NAD+ + ?
-
 ?
NADH + hexaammineruthenium-III-chloride
-
 Escherichia coli MWC215 NAD+ + ?
-
 ?
NADH + ubiquinone
-
 Escherichia coli NAD+ + ubiquinol
-
 ?
NADH + ubiquinone
-
 Escherichia coli MWC215 NAD+ + ubiquinol
-
 ?
NADH + ubiquinone-8
-
 Escherichia coli NAD+ + ubiquinol-8
-
 ?
NADH + ubiquinone-8
-
 Escherichia coli MWC215 NAD+ + ubiquinol-8
-
 ?

Synonyms

Synonyms Comment Organism
complex I
-
 Escherichia coli
NADH:ubiquinone oxidoreductase
-
 Escherichia coli
NDH-1
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1500
-
 hexaammineruthenium-III-chloride
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Escherichia coli
NADH
-
 Escherichia coli