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Literature summary for 7.1.1.2 extracted from
- A role for native lipids in the stabilization and two-dimensional crystallization of the Escherichia coli NADH-ubiquinone oxidoreductase (complex I) (2003), J. Biol. Chem., 278, 19483-19491.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| two different two-dimensionmal crystal forms with p2 and p3 symmetry, are obtained using lipid containing native Escherichia coli extract | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| 4°C, complex I is intact for about 2 days, stable for 3-5 days with inclusion of 10 mM CaCl2 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 50 mM or greater | Escherichia coli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 5-10 mM optimal for activity | Escherichia coli | |
| NaCl | optimal concentration between 20 mM and 50 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 600000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
Bl21 strain | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | native lipids play an important role in the activation, stabilization and, as a sonsequence, crystallization of purified complex I | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 57.9 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, complex I is intact for about 2 days, stable for 3-5 days with inclusion of 10 mM CaCl2 | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH-ubiquinone oxidoreductase (complex I) | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Escherichia coli | |
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