Literature summary for 7.1.1.2 extracted from

Grivennikova, V.G.; Roth, R.; Zakharova, N.V.; Hagerhall, C.; Vinogradov, A.D.
The mitochondrial and prokaryotic proton-translocating NADH:ubiquinone oxidoreductases: similarities and dissimilarities of the quinone-junction sites (2003), Biochim. Biophys. Acta, 1607, 79-90.

Search for more literature for 7.1.1.2

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP-ribose	competitive with NADH	Bos taurus
ADP-ribose	competitive with NADH	Paracoccus denitrificans
rotenone	-	Bos taurus
rotenone	inhibition of NADH oxidation, NAD+ reduction is not inhibited by 0.001 mM rotenone	Paracoccus denitrificans
rotenone	-	Rhodobacter capsulatus
Triton X-100	-	Bos taurus
Triton X-100	-	Paracoccus denitrificans
Triton X-100	-	Rhodobacter capsulatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0014	-	NADH	coupled oxidase reaction, pH 8.0, 25°C	Bos taurus
0.002	-	NADH	coupled oxidase reaction, pH 8.0, 25°C	Paracoccus denitrificans
0.0022	-	NADH	uncoupled oxidase reaction, pH 8.0, 25°C	Bos taurus
0.006	-	NADH	uncoupled oxidase reaction, pH 8.0, 25°C	Rhodobacter capsulatus
0.007	-	NAD+	pH 8.0, 25°C, succinate supported NAD+ reduction	Bos taurus
0.007	-	NADH	uncoupled oxidase reaction, pH 8.0, 25°C	Paracoccus denitrificans
0.01	-	coenzyme Q1	pH 8.0, 25°C	Bos taurus
0.015	-	coenzyme Q1	pH 8.0, 25°C	Rhodobacter capsulatus
0.02	-	coenzyme Q1	pH 8.0, 25°C	Paracoccus denitrificans
0.27	-	NAD+	pH 8.0, 25°C, succinate supported NAD+ reduction	Paracoccus denitrificans

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Paracoccus denitrificans	-	-	-
Rhodobacter capsulatus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + H+ + coenzyme Q1	-	Paracoccus denitrificans	NAD+ + reduced coenzyme Q1	-	?
NADH + H+ + coenzyme Q1	-	Bos taurus	NAD+ + reduced coenzyme Q1	-	?
NADH + H+ + coenzyme Q1	-	Rhodobacter capsulatus	NAD+ + reduced coenzyme Q1	-	?

Synonyms

Synonyms	Comment	Organism
complex I	-	Paracoccus denitrificans
complex I	-	Bos taurus
complex I	-	Rhodobacter capsulatus
NADH:ubiquinone oxidoreductase	-	Paracoccus denitrificans
NADH:ubiquinone oxidoreductase	-	Bos taurus
NADH:ubiquinone oxidoreductase	-	Rhodobacter capsulatus

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Paracoccus denitrificans
NADH	-	Bos taurus
NADH	-	Rhodobacter capsulatus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000001	-	rotenone	pH 8.0, 25°C, NADH oxidase reaction	Bos taurus
0.00002	-	rotenone	pH 8.0, 25°C, NAD+ reduction	Bos taurus
0.00015	-	rotenone	pH 8.0, 25°C, NADH oxidase reaction	Rhodobacter capsulatus
0.0005	-	rotenone	pH 8.0, 25°C, NADH oxidase reaction	Paracoccus denitrificans
0.007	-	Triton X-100	pH 8.0, 25°C, NADH oxidase reaction	Bos taurus
0.01	-	Triton X-100	pH 8.0, 25°C, NAD+ reduction	Paracoccus denitrificans
0.025	-	ADP-ribose	pH 8.0, 25°C	Bos taurus
0.025	-	Triton X-100	pH 8.0, 25°C, NADH oxidase reaction	Rhodobacter capsulatus
0.04	-	Triton X-100	pH 8.0, 25°C, NAD+ reduction	Bos taurus
0.05	-	Triton X-100	pH 8.0, 25°C, NADH oxidase reaction	Paracoccus denitrificans
0.08	-	ADP-ribose	pH 8.0, 25°C	Paracoccus denitrificans

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Release 2023.1 (January 2023)