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Literature summary for 7.1.1.2 extracted from
- Purification of NADH-ferricyanide dehydrogenase and NADH-quinone reductase from Escherichia coli membranes and their roles in the respiratory chain (1989), Biochim. Biophys. Acta, 977, 62-69.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| liponox DCH | required at 0.02% for optimum activity | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| liponox DCH | inhibitory at concentrations higher than 0.02% | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0438 | - |
NADH | enzyme activated before assay, electron acceptor: ferricyanide | Escherichia coli | |
| 0.0452 | - |
NADH | electron acceptor: ferricyanide | Escherichia coli | |
| 0.0483 | - |
NADH | enzyme activated before assay, electron acceptor: ubiquinone-1 | Escherichia coli | |
| 0.0965 | - |
NADH | electron acceptor: ubiquinone-1 | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using column chromatography on DEAE-Sephacel and DEAE-5PW | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADH | - |
Escherichia coli | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| NADH + menadione | menadione is a poor electron acceptor | Escherichia coli | NAD+ + menadiol | - |
? | |
| NADH + oxidized 2,6-dichlorophenolindophenol + H+ | 2,6-dichlorophenolindophenol is a poor electron acceptor | Escherichia coli | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| NADH + ubiquinone-1 | - |
Escherichia coli | NAD+ + ubiquinol-1 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 2.97 nmol per mg of enzyme | Escherichia coli | |
| NADH | - |
Escherichia coli | |
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