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Literature summary for 7.1.1.2 extracted from
- Purification and properties of NADH dehydrogenase from a thermoacidophilic archaebacterium, Sulfolobus acidocaldarius (1987), J. Biochem., 102, 255-262.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 0.1 mM, about 2fold stimulation | Sulfolobus acidocaldarius |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Sulfolobus acidocaldarius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
naphthoquinone | - |
Sulfolobus acidocaldarius | |
| 0.1 | - |
benzoquinone | - |
Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Sulfolobus acidocaldarius |
| 95000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + benzoquinone | - |
Sulfolobus acidocaldarius | NAD+ + benzoquinol | - |
? | |
| NADH + caldariellaquinone | - |
Sulfolobus acidocaldarius | NAD+ + caldariellaquinol | - |
? | |
| NADH + naphthoquinone | - |
Sulfolobus acidocaldarius | NAD+ + naphthoquinol | - |
? | |
| NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Sulfolobus acidocaldarius | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| NADH + ubiquinone | - |
Sulfolobus acidocaldarius | NAD+ + ubiquinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 4, stable for at least 1 h | Sulfolobus acidocaldarius |
| 100 | - |
pH 7, 15 min, 30% loss of activity | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
reaction with ferricyanide | Sulfolobus acidocaldarius |
| 7.5 | - |
reaction with 2,6-dichlorophenolindophenol | Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | 50% of maximal activity at pH 3.5 and 7.0, reaction with ferricyanide | Sulfolobus acidocaldarius |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
25°C, stable for at least 1 h | Sulfolobus acidocaldarius |
| 7 | - |
100°C, 15 min, 30% loss of activity | Sulfolobus acidocaldarius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | enzyme contains 1.4 mol flavin per mol of enzyme | Sulfolobus acidocaldarius | |
| NADH | - |
Sulfolobus acidocaldarius | |
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