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Literature summary for 7.1.1.2 extracted from
- Novel FMN-containing rotenone-insensitive NADH dehydrogenase from Trypanosoma brucei mitochondria: isolation and characterization (2002), Biochemistry, 41, 3065-3072.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphenyl iodonium | maximal inhibition after preincubation with NADH, more complete inhibition with the more hydrophobic electron acceptors such as ubiquinone-1 or ubiquinone-2 as electron acceptor compared to the more hydrophilic ones, such as ubiquinone-0 or dichloroindophenol | Trypanosoma brucei |  |
| flavone | insensitive to | Trypanosoma brucei | |
| rotenone | rotenone-insensitive enzyme | Trypanosoma brucei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | inner membrane | Trypanosoma brucei | 31966 | - |
| mitochondrion | inner membrane | Trypanosoma brucei | 5739 | - |
| mitochondrion | loosly bound to inner mitochondrial membrane | Trypanosoma brucei | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Trypanosoma brucei |
| 65000 | - |
gel filtration | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma brucei |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| NADH + ubiquinone + 6 H+[side 1] = NAD+ + ubiquinol + 7 H+[side 2] | ping-pong mechanism | Trypanosoma brucei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | procyclic form of the organism | Trypanosoma brucei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + O2 | - |
Trypanosoma brucei | NAD+ + superoxide radical | - |
? | |
| NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Trypanosoma brucei | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| NADH + ubiquinone | - |
Trypanosoma brucei | NAD+ + ubiquinol | - |
? | |
| NADH + ubiquinone-0 | - |
Trypanosoma brucei | NAD+ + ubiquinol-0 | - |
? | |
| NADH + ubiquinone-1 | - |
Trypanosoma brucei | NAD+ + ubiquinol-1 | - |
? | |
| NADH + ubiquinone-2 | - |
Trypanosoma brucei | NAD+ + reduced ubiquinol-2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, SDS-PAGE | Trypanosoma brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Trypanosoma brucei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | stable | Trypanosoma brucei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | enzyme contains noncovalently bound FMN | Trypanosoma brucei | |
| NADH | - |
Trypanosoma brucei | |
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