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Literature summary for 7.1.1.2 extracted from

  • Soole, K.L.; Dry, I.B.; Wiskich, J.T.
    Partial purification and characterization of complex I, NADH:ubiquinone reductase, from the inner membrane of beetroot mitochondria (1991), Plant Physiol., 98, 588-594.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.1.2

Inhibitors

Inhibitors Comment Organism Structure
Mersalyl 0.3 mM, 77% inhibition of NADH-ubiquinone-1 reductase activity, 96% inhibition of NADH-potassium ferricyanide reductase activity Beta vulgaris
NAD+ 4 mM, 19% inhibition of NADH-ubiquinone-1 reductase activity Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 NADH reaction with ubiquinone-1 Beta vulgaris
0.055
-
 ferricyanide
-
 Beta vulgaris
0.143
-
 ubiquinone-1
-
 Beta vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane inner membrane Beta vulgaris 31966
-
mitochondrion inner membrane Beta vulgaris 5739
-

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Beta vulgaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
-
 Beta vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Beta vulgaris 2 ferrocyanide + NAD+ + H+
-
 ?
additional information no transhydrogenase activity with NADPH Beta vulgaris ?
-
 ?
NADH + ubiquinone
-
 Beta vulgaris NAD+ + ubiquinol
-
 ?
NADPH + H+ + ubiquinone-1 at a slow rate Beta vulgaris NADP+ + ubiquinol-1
-
 ?

Subunits

Subunits Comment Organism
More isolated complex contains 14 major polypeptides Beta vulgaris

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Beta vulgaris