Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A398C | the mutant with wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
A432C | the mutant shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
D392C | the mutant shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
G408C | the mutant with wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
G430C | the mutant shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
I406C | the mutant with 450% of wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
M409C | the mutant with 75% of wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
S404C | the mutant with 75% of wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
T393C | the mutant shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
V411C | the mutant with 125% of wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
Y431C | the mutant with 450% of wild type activity shows increased ratios between the rates of the forward and reverse reactions, thus approaching that of the wild type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | Escherichia coli | - |
NADP+ + NADH + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, and butyl Toyopearl column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | - |
Escherichia coli | NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Escherichia coli | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
? | |
thio-NADP+ + NADH + H+[side 2] | - |
Escherichia coli | thio-NADPH + NAD+ + H+[side 1] | - |
? |