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Literature summary for 7.1.1.1 extracted from

  • Hutton, M.; Day, J.M.; Bizouarn, T.; Jackson, J.B.
    Kinetic resolution of the reaction catalysed by proton-translocating transhydrogenase from Escherichia coli as revealed by experiments with analogues of the nucleotide substrates (1994), Eur. J. Biochem., 219, 1041-1051.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM83 cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
N,N'-dicyclohexylcarbodiimide modification of the enzyme with N,N'-dicyclohexylcarbodiimide leads to inhibition of the rate of release of NADP+ and NADPH from the enzyme, but has a much smaller effect on the binding and release of NAD+, NADH and their analogues and on the interconversion of the ternary complexes of the enzyme with its substrates Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
1 * 54000 + 1 * 49000, SDS-PAGE Escherichia coli
54000
-
1 * 54000 + 1 * 49000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + NAD+ + H+[side 1] Escherichia coli
-
NADP+ + NADH + H+[side 2]
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column chromatography and DEAE-Trisacryl M column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
with oxidized acetyl pyridine adenine dinucleotide and deamino-NADPH as substrates, at pH 6.0 and 25°C Escherichia coli
0.47
-
with oxidized acetyl pyridine adenine dinucleotide and 1-N6-etheno-NADPH as substrates, at pH 6.0 and 25°C Escherichia coli
1.46
-
with oxidized acetyl pyridine adenine dinucleotide and NADPH as substrates, at pH 6.0 and 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,N6-etheno-NADPH + NAD+ + H+[side 1]
-
Escherichia coli 1,N6-etheno-NADP+ + NADH + H+[side 2]
-
r
1,N6-etheno-NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
Escherichia coli 1,N6-etheno-NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
r
deamino-NADPH + NAD+ + H+[side 1]
-
Escherichia coli deamino-NADP+ + NADH + H+[side 2]
-
r
deamino-NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
Escherichia coli deamino-NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
r
NADPH + NAD+ + H+[side 1]
-
Escherichia coli NADP+ + NADH + H+[side 2]
-
r
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
Escherichia coli NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
r

Subunits

Subunits Comment Organism
heterodimer 1 * 54000 + 1 * 49000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
H+-transhydrogenase
-
Escherichia coli
proton-translocating transhydrogenase
-
Escherichia coli