Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging-drop method, crystal structures of the NAD(H)-binding domain I of transhydrogenase in the absence as well as in the presence of oxidized and reduced substrate. The structures are determined at 1.9-2.0 A resolution | Escherichia coli |
NAD(H)-binding domain I in the absence and in the presence of NAD+ or NADH, hanging drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M tri-sodium citrate dihydrate (pH 5.6) and 30% (w/v) PEG 4000 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+/out + NADH + NADP+ | Escherichia coli | the enzyme couples the redox reaction between NAD(H) and NADP(H) to the inward transport of protons across a membrane | H+/in + NAD+ + NADPH | - |
? | |
NADPH + NAD+ + H+[side 1] | Escherichia coli | - |
NADP+ + NADH + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P07001 | - |
- |
Escherichia coli | P07001 | subunit alpha | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+/out + NADH + NADP+ | the enzyme couples the redox reaction between NAD(H) and NADP(H) to the inward transport of protons across a membrane | Escherichia coli | H+/in + NAD+ + NADPH | - |
? | |
NADPH + NAD+ + H+[side 1] | - |
Escherichia coli | NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Escherichia coli | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r |
Synonyms | Comment | Organism |
---|---|---|
proton-pumping membrane protein transhydrogenase | - |
Escherichia coli |