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Literature summary for 7.1.1.1 extracted from

  • Johansson, T.; Oswald, C.; Pedersen, A.; Toernroth, S.; Okvist, M.; Karlsson, B.G.; Rydstroem, J.; Krengel, U.
    X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli (2005), J. Mol. Biol., 352, 299-312.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop method, crystal structures of the NAD(H)-binding domain I of transhydrogenase in the absence as well as in the presence of oxidized and reduced substrate. The structures are determined at 1.9-2.0 A resolution Escherichia coli
NAD(H)-binding domain I in the absence and in the presence of NAD+ or NADH, hanging drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M tri-sodium citrate dihydrate (pH 5.6) and 30% (w/v) PEG 4000 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H+/out + NADH + NADP+ Escherichia coli the enzyme couples the redox reaction between NAD(H) and NADP(H) to the inward transport of protons across a membrane H+/in + NAD+ + NADPH
-
?
NADPH + NAD+ + H+[side 1] Escherichia coli
-
NADP+ + NADH + H+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P07001
-
-
Escherichia coli P07001 subunit alpha
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+/out + NADH + NADP+ the enzyme couples the redox reaction between NAD(H) and NADP(H) to the inward transport of protons across a membrane Escherichia coli H+/in + NAD+ + NADPH
-
?
NADPH + NAD+ + H+[side 1]
-
Escherichia coli NADP+ + NADH + H+[side 2]
-
?
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
Escherichia coli NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
r

Synonyms

Synonyms Comment Organism
proton-pumping membrane protein transhydrogenase
-
Escherichia coli