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Literature summary for 7.1.1.1 extracted from

  • Yamaguchi, M.; Stout, C.D.
    Essential glycine in the proton channel of Escherichia coli transhydrogenase (2003), J. Biol. Chem., 278, 45333-45339.
    View publication on PubMed
Search for more literature for 7.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MC4100TH- Escherichia coli

Protein Variants

Protein Variants Comment Organism
G132A in domain dII, no effect on wild type reverse activity Escherichia coli
G138A in domain dII, 57% of wild type reverse activity Escherichia coli
G226A in domain dII, 50% of wild type reverse activity Escherichia coli
G233A in domain dII, 49% of wild type reverse activity Escherichia coli
G245A in domain dII, no effect of wild type reverse activity Escherichia coli
G249A in domain dII, 79% wild type reverse activity Escherichia coli
G252A in domain dII, 2.6% of wild type reverse activity Escherichia coli
G252C in domain dII, 1.9% of wild type reverse activity Escherichia coli
G252S in domain dII, 2.4% of wild type reverse activity Escherichia coli
G252T in domain dII, 2.3% of wild type reverse activity Escherichia coli
G252V in domain dII, 2.5% of wild type reverse activity Escherichia coli
G95A in domain dII, 56% of wild type reverse activity Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + NADP+ Escherichia coli links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, provides NADPH for the reduction of glutathione, reverse reaction results in outward proton translocation and creation of a proton motive force NAD+ + NADPH
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + NADP+
-
 Escherichia coli NAD+ + NADPH
-
 r
NADH + NADP+ links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, provides NADPH for the reduction of glutathione, reverse reaction results in outward proton translocation and creation of a proton motive force Escherichia coli NAD+ + NADPH
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
 Escherichia coli
NAD(P)H
-
 Escherichia coli