Any feedback?
Literature summary for 7.1.1.1 extracted from
- A change in ionization of the NADP(H)-binding component (dIII) if proton-translocating transhydrogenase regulates both hydride transfer and nucleotide release (2001), Eur. J. Biochem., 268, 1430-1438.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Rhodospirillum rubrum |
| expression of domain I and of E155W and Y171W mutant domain III | Rhodospirillum rubrum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E155W | mutation in domain III, similar catalytic activities as wild-type, used for tryptophan fluorescence measurements | Rhodospirillum rubrum |
| E155W | mutant of the NADP(H)-binding component | Rhodospirillum rubrum |
| Y171W | mutation in domain III, similar catalytic activities as wild-type, used for tryptophan fluorescence measurements | Rhodospirillum rubrum |
| Y171W | the mutant shows wild type activity | Rhodospirillum rubrum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + NAD+ + H+[side 1] | Rhodospirillum rubrum | - |
NADP+ + NADH + H+[side 2] | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodospirillum rubrum |
| recombinant domain I and recombinant E155W and Y171W mutant domain III | Rhodospirillum rubrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + NAD+ + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + NADH + H+[side 2] | - |
? | |
| NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| proton-translocating transhydrogenase | - |
Rhodospirillum rubrum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rhodospirillum rubrum | |
| NADH | - |
Rhodospirillum rubrum | |
| NADP+ | - |
Rhodospirillum rubrum | |
| NADPH | - |
Rhodospirillum rubrum | |
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Release 2023.1 (January 2023)
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