Protein Variants | Comment | Organism |
---|---|---|
H337A | residue His337 is the site of covalent guanylylation, the mutation abolishes 3'-phosphate/5'-OH RNA ligation | Escherichia coli |
H337N | residue His337 is the site of covalent guanylylation, the mutation abolishes 3'-phosphate/5'-OH RNA ligation | Escherichia coli |
H337Q | residue His337 is the site of covalent guanylylation, the mutation abolishes 3'-phosphate/5'-OH RNA ligation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P46850 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m | - |
Escherichia coli | (ribonucleotide)n+m + GMP | - |
? | |
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate | substrate for ligation is a 20-mer RNA strand with 5'-OH and 3'-monophosphate ends | Escherichia coli | (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine | - |
? | |
GTP + [RNA ligase]-L-histidine | - |
Escherichia coli | 5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + diphosphate | - |
? | |
additional information | RtcB executes a three-step ligation pathway, consisting of reaction of His337 of the enzyme with GTP to form a covalent RtcB-(histidinyl-N)-GMP intermediate, transfer of guanylate to a polynucleotide 3'-phosphate to form a polynucleotide-(3')pp(5')G intermediate, and (iii) attack of a 5'-OH on the N(3')pp(5')G end to form the splice junction. Presence of GTP is required | Escherichia coli | ? | - |
? |