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Literature summary for 6.5.1.8 extracted from

  • Chakravarty, A.K.; Subbotin, R.; Chait, B.T.; Shuman, S.
    RNA ligase RtcB splices 3-phosphate and 5-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3)pp(5)G intermediates (2012), Proc. Natl. Acad. Sci. USA, 109, 6072-6077.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
H337A residue His337 is the site of covalent guanylylation, the mutation abolishes 3'-phosphate/5'-OH RNA ligation Escherichia coli
H337N residue His337 is the site of covalent guanylylation, the mutation abolishes 3'-phosphate/5'-OH RNA ligation Escherichia coli
H337Q residue His337 is the site of covalent guanylylation, the mutation abolishes 3'-phosphate/5'-OH RNA ligation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P46850
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m
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Escherichia coli (ribonucleotide)n+m + GMP
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?
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate substrate for ligation is a 20-mer RNA strand with 5'-OH and 3'-monophosphate ends Escherichia coli (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine
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?
GTP + [RNA ligase]-L-histidine
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Escherichia coli 5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + diphosphate
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?
additional information RtcB executes a three-step ligation pathway, consisting of reaction of His337 of the enzyme with GTP to form a covalent RtcB-(histidinyl-N)-GMP intermediate, transfer of guanylate to a polynucleotide 3'-phosphate to form a polynucleotide-(3')pp(5')G intermediate, and (iii) attack of a 5'-OH on the –N(3')pp(5')G end to form the splice junction. Presence of GTP is required Escherichia coli ?
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