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Literature summary for 6.5.1.8 extracted from

  • Englert, M.; Sheppard, K.; Aslanian, A.; Yates, J.R.; Soell, D.
    Archaeal 3-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation (2011), Proc. Natl. Acad. Sci. USA, 108, 1290-1295.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
C100A inactive for ligation reaction Pyrobaculum aerophilum
H205A inactive for ligation reaction Pyrobaculum aerophilum
H236A inactive for ligation reaction Pyrobaculum aerophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Pyrobaculum aerophilum
Zn2+ required, assay at 0.5 mM Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
gel filtration Pyrococcus horikoshii
55000
-
gel filtration Methanopyrus kandleri
55000
-
gel filtration Pyrobaculum aerophilum

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri Q8TUS2
-
-
Methanopyrus kandleri DSM 6324 Q8TUS2
-
-
Pyrobaculum aerophilum Q8ZY09
-
-
Pyrobaculum aerophilum DSM 7523 Q8ZY09
-
-
Pyrococcus horikoshii O59245
-
-
Pyrococcus horikoshii DSM 12428 O59245
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease Pyrococcus horikoshii (ribonucleotide)n+m
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease Pyrobaculum aerophilum (ribonucleotide)n+m RtcB-catalyzed ligation incorporates the phosphate of the cyclic phosphate into the phosphodiester bond. ATP or GTP is not required ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease Pyrobaculum aerophilum DSM 7523 (ribonucleotide)n+m RtcB-catalyzed ligation incorporates the phosphate of the cyclic phosphate into the phosphodiester bond. ATP or GTP is not required ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease Pyrococcus horikoshii DSM 12428 (ribonucleotide)n+m
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Pyrococcus horikoshii
monomer 1 * 55000, SDS-PAGE Methanopyrus kandleri
monomer 1 * 55000, SDS-PAGE Pyrobaculum aerophilum

Synonyms

Synonyms Comment Organism
3'-P RNL
-
Pyrococcus horikoshii
3'-P RNL
-
Methanopyrus kandleri
3'-P RNL
-
Pyrobaculum aerophilum