Protein Variants | Comment | Organism |
---|---|---|
C100A | inactive for ligation reaction | Pyrobaculum aerophilum |
H205A | inactive for ligation reaction | Pyrobaculum aerophilum |
H236A | inactive for ligation reaction | Pyrobaculum aerophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Pyrobaculum aerophilum | |
Zn2+ | required, assay at 0.5 mM | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Pyrococcus horikoshii |
55000 | - |
gel filtration | Methanopyrus kandleri |
55000 | - |
gel filtration | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | Q8TUS2 | - |
- |
Methanopyrus kandleri DSM 6324 | Q8TUS2 | - |
- |
Pyrobaculum aerophilum | Q8ZY09 | - |
- |
Pyrobaculum aerophilum DSM 7523 | Q8ZY09 | - |
- |
Pyrococcus horikoshii | O59245 | - |
- |
Pyrococcus horikoshii DSM 12428 | O59245 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m | generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease | Pyrococcus horikoshii | (ribonucleotide)n+m | - |
? | |
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m | generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease | Pyrobaculum aerophilum | (ribonucleotide)n+m | RtcB-catalyzed ligation incorporates the phosphate of the cyclic phosphate into the phosphodiester bond. ATP or GTP is not required | ? | |
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m | generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease | Pyrobaculum aerophilum DSM 7523 | (ribonucleotide)n+m | RtcB-catalyzed ligation incorporates the phosphate of the cyclic phosphate into the phosphodiester bond. ATP or GTP is not required | ? | |
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m | generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease | Pyrococcus horikoshii DSM 12428 | (ribonucleotide)n+m | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Pyrococcus horikoshii |
monomer | 1 * 55000, SDS-PAGE | Methanopyrus kandleri |
monomer | 1 * 55000, SDS-PAGE | Pyrobaculum aerophilum |
Synonyms | Comment | Organism |
---|---|---|
3'-P RNL | - |
Pyrococcus horikoshii |
3'-P RNL | - |
Methanopyrus kandleri |
3'-P RNL | - |
Pyrobaculum aerophilum |