Cloned (Comment) | Organism |
---|---|
exprression in Escherichia coli | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel fitlration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P46850 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | substrate mimicks the broken tRNAGlu(UUC) anticodon stem-loop generated by Kluyveromyces lactis gamma-toxin, leaving 2',3'-cyclic phosphate and 5'-OH ends | Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | overall reaction | ? | |
additional information | RtcB seals broken tRNA-like stem-loop structures with 2',3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3',5'-phosphodiester | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Escherichia coli |