Cloned (Comment) | Organism |
---|---|
- |
Sulfophobococcus zilligii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | both Mg2+ and Mn2+ support the maximum enzyme activity at a concentration of 5 mM and 7 mM respectively | Sulfophobococcus zilligii | |
Mn2+ | both Mg2+ and Mn2+ support the maximum enzyme activity at a concentration of 5 mM and 7 mM respectively | Sulfophobococcus zilligii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, enzyme with His-tag, SDS-PAGE | Sulfophobococcus zilligii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Sulfophobococcus zilligii | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | AMP + phosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Sulfophobococcus zilligii | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Sulfophobococcus zilligii DSM Z 11193 | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Sulfophobococcus zilligii | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | GMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Sulfophobococcus zilligii DSM Z 11193 | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | GMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfophobococcus zilligii | - |
- |
- |
Sulfophobococcus zilligii DSM Z 11193 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfophobococcus zilligii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | Sulfophobococcus zilligii | AMP + phosphate + (deoxyribonucleotide)n+m | - |
? | |
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | 72% nick-closing activity as compared with ATP | Sulfophobococcus zilligii | AMP + phosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | nick-closing activity | Sulfophobococcus zilligii | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | Sulfophobococcus zilligii | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | nick-closing activity | Sulfophobococcus zilligii DSM Z 11193 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | Sulfophobococcus zilligii DSM Z 11193 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | Sulfophobococcus zilligii | GMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | 63% nick-closing activity as compared with ATP | Sulfophobococcus zilligii | GMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions | Sulfophobococcus zilligii DSM Z 11193 | GMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | 63% nick-closing activity as compared with ATP | Sulfophobococcus zilligii DSM Z 11193 | GMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
additional information | activity with CTP, TTP or NAD+ is lees than 5% compared to the activity with ATP | Sulfophobococcus zilligii | ? | - |
? | |
additional information | activity with CTP, TTP or NAD+ is lees than 5% compared to the activity with ATP | Sulfophobococcus zilligii DSM Z 11193 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, enzyme with His-tag, SDS-PAGE | Sulfophobococcus zilligii |
Synonyms | Comment | Organism |
---|---|---|
Szi DNA ligase | - |
Sulfophobococcus zilligii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Sulfophobococcus zilligii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 90 | 65°C: about 40% of maximal activity, 90°C: about 40% of maximal activity | Sulfophobococcus zilligii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
the enzyme reaches its maximum activity when incubated at 75°C for 1 h and gradually loses its activity thereafter | Sulfophobococcus zilligii |
95 | - |
half-life: 2.6 h | Sulfophobococcus zilligii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Sulfophobococcus zilligii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 75% of maximal activity, pH 8.0: about 55% of maximal activity | Sulfophobococcus zilligii |