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Literature summary for 6.5.1.7 extracted from

  • Sun, Y.; Seo, M.S.; Kim, J.H.; Kim, Y.J.; Kim, G.A.; Lee, J.I.; Lee, J.H.; Kwon, S.T.
    Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization (2008), Environ. Microbiol., 10, 3212-3224.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Sulfophobococcus zilligii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ both Mg2+ and Mn2+ support the maximum enzyme activity at a concentration of 5 mM and 7 mM respectively Sulfophobococcus zilligii
Mn2+ both Mg2+ and Mn2+ support the maximum enzyme activity at a concentration of 5 mM and 7 mM respectively Sulfophobococcus zilligii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, enzyme with His-tag, SDS-PAGE Sulfophobococcus zilligii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Sulfophobococcus zilligii multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions AMP + phosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Sulfophobococcus zilligii multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Sulfophobococcus zilligii DSM Z 11193 multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Sulfophobococcus zilligii multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions GMP + diphosphate + (deoxyribonucleotide)n+m
-
?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Sulfophobococcus zilligii DSM Z 11193 multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions GMP + diphosphate + (deoxyribonucleotide)n+m
-
?

Organism

Organism UniProt Comment Textmining
Sulfophobococcus zilligii
-
-
-
Sulfophobococcus zilligii DSM Z 11193
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfophobococcus zilligii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions Sulfophobococcus zilligii AMP + phosphate + (deoxyribonucleotide)n+m
-
?
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m 72% nick-closing activity as compared with ATP Sulfophobococcus zilligii AMP + phosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Sulfophobococcus zilligii AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions Sulfophobococcus zilligii AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Sulfophobococcus zilligii DSM Z 11193 AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions Sulfophobococcus zilligii DSM Z 11193 AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions Sulfophobococcus zilligii GMP + diphosphate + (deoxyribonucleotide)n+m
-
?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m 63% nick-closing activity as compared with ATP Sulfophobococcus zilligii GMP + diphosphate + (deoxyribonucleotide)n+m
-
?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m multiple cofactor specificity of the DNA ligase from Sulfophobococcus zilligii may possibly be attributed to the ease of decomposition of ATP at the high temperatures. Due to limited amount of ATP, other NTPs might have been employed as alternative energy sources. This unique biochemical feature seems to have evolved to permit survival under unusual conditions Sulfophobococcus zilligii DSM Z 11193 GMP + diphosphate + (deoxyribonucleotide)n+m
-
?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m 63% nick-closing activity as compared with ATP Sulfophobococcus zilligii DSM Z 11193 GMP + diphosphate + (deoxyribonucleotide)n+m
-
?
additional information activity with CTP, TTP or NAD+ is lees than 5% compared to the activity with ATP Sulfophobococcus zilligii ?
-
?
additional information activity with CTP, TTP or NAD+ is lees than 5% compared to the activity with ATP Sulfophobococcus zilligii DSM Z 11193 ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, enzyme with His-tag, SDS-PAGE Sulfophobococcus zilligii

Synonyms

Synonyms Comment Organism
Szi DNA ligase
-
Sulfophobococcus zilligii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Sulfophobococcus zilligii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 90 65°C: about 40% of maximal activity, 90°C: about 40% of maximal activity Sulfophobococcus zilligii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
the enzyme reaches its maximum activity when incubated at 75°C for 1 h and gradually loses its activity thereafter Sulfophobococcus zilligii
95
-
half-life: 2.6 h Sulfophobococcus zilligii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sulfophobococcus zilligii

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 75% of maximal activity, pH 8.0: about 55% of maximal activity Sulfophobococcus zilligii