BRENDA - Enzyme Database
show all sequences of 6.5.1.5

GTP-dependent RNA 3'-terminal phosphate cyclase from the hyperthermophilic archaeon Pyrococcus furiosus

Sato, A.; Soga, T.; Igarashi, K.; Takesue, K.; Tomita, M.; Kanai, A.; Genes Cells 16, 1190-1199 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression of the recombinant C-terminal His-tagged protein in the Escherichia coli strain BL21(DE3)pLysS
Pyrococcus furiosus
Inhibitors
Inhibitors
Commentary
Organism
Structure
GMP
-
Pyrococcus furiosus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
activation, Mn2+ can substitute for Mg2+
Pyrococcus furiosus
Mn2+
activation, Mn2+ can substitute for Mg2+
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
x * 37000, SDS-PAGE
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
GTP + RNA 3'-terminal-phosphate
Pyrococcus furiosus
the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pyrococcus furiosus
Q8U0N7
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
GTP + RNA 3'-terminal-phosphate
the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase
722334
Pyrococcus furiosus
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
GTP + RNA 3'-terminal-phosphate
the enzyme utilizes GTP approximately 10times more effectively than ATP. UTP and CTP have no effect on activity. Oligoribonucleotide substrates of different lengths (4-, 8- and 12-mers) are tested, the 4-mer is the most efficient substrate
722334
Pyrococcus furiosus
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE
Pyrococcus furiosus
Synonyms
Synonyms
Commentary
Organism
Pf-Rtc
-
Pyrococcus furiosus
PF1549
-
Pyrococcus furiosus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
22
95
similar activity in the range 22°C-75°C, much higher activity at 95°C
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of the recombinant C-terminal His-tagged protein in the Escherichia coli strain BL21(DE3)pLysS
Pyrococcus furiosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
GMP
-
Pyrococcus furiosus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
activation, Mn2+ can substitute for Mg2+
Pyrococcus furiosus
Mn2+
activation, Mn2+ can substitute for Mg2+
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
x * 37000, SDS-PAGE
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
GTP + RNA 3'-terminal-phosphate
Pyrococcus furiosus
the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
GTP + RNA 3'-terminal-phosphate
the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase
722334
Pyrococcus furiosus
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
GTP + RNA 3'-terminal-phosphate
the enzyme utilizes GTP approximately 10times more effectively than ATP. UTP and CTP have no effect on activity. Oligoribonucleotide substrates of different lengths (4-, 8- and 12-mers) are tested, the 4-mer is the most efficient substrate
722334
Pyrococcus furiosus
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE
Pyrococcus furiosus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
22
95
similar activity in the range 22°C-75°C, much higher activity at 95°C
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pyrococcus furiosus
General Information
General Information
Commentary
Organism
physiological function
the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase
Pyrococcus furiosus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase
Pyrococcus furiosus
Other publictions for EC 6.5.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722334
Sato
GTP-dependent RNA 3'-terminal ...
Pyrococcus furiosus
Genes Cells
16
1190-1199
2011
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