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Literature summary for 6.5.1.4 extracted from

  • Tanaka, N.; Shuman, S.
    Structure-activity relationships in human RNA 3-phosphate cyclase (2009), RNA, 15, 1865-1874.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D297A enzyme inactive Homo sapiens
E14A activity very low, only 4% of RNA is cyclized compared to 95% with wild-type enzyme Homo sapiens
H320A enzyme inactive Homo sapiens
H326A almost same activiy like wild-type enzyme Homo sapiens
H52A half activity compared to wild-type enzyme Homo sapiens
Q51A almost same activiy like wild-type enzyme Homo sapiens
R21A enzyme inactive Homo sapiens
R40A enzyme inactive Homo sapiens
R43A enzyme inactive Homo sapiens
Y294A enzyme inactive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 ATP
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli P46849
-
-
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AAAAUAAAAGpCp
-
 Homo sapiens AMP + diphosphate + AAAAUAAAAGpC-2',3'-cyclic phosphate
-
 ir
ATP + AAAAUAAAAGpCp
-
 Escherichia coli AMP + diphosphate + AAAAUAAAAGpC-2',3'-cyclic phosphate
-
 ir

Synonyms

Synonyms Comment Organism
RNA 3'-phosphate cyclase
-
 Homo sapiens
RNA 3'-phosphate cyclase
-
 Escherichia coli
Rtc
-
 Homo sapiens
Rtc
-
 Escherichia coli
Rtc1
-
 Homo sapiens
RtcA
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism synthesis of RNA 2',3' cyclic phosphate ends via an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of Rtc with ATP to form a covalent Rtc-(histidinyl-N)-AMP intermediate and release PPi, transfer of AMP from Rtc1 to an RNA 3'-phosphate to form an RNA(3')pp(5')A intermediate; and attack by the terminal nucleoside O2' on the 3'-phosphate to form an RNA 2',3' cyclic phosphate product and release AMP Escherichia coli
metabolism synthesis of RNA 2',3'-cyclic phosphate ends via an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of Rtc with ATP to form a covalent Rtc-(histidinyl-N)-AMP intermediate and release diphosphate, transfer of AMP from Rtc1 to an RNA 3'-phosphate to form an RNA(3')pp(5')A intermediate; and attack by the terminal nucleoside O2' on the 3'-phosphate to form an RNA 2',3'-cyclic phosphate product and release AMP Homo sapiens