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Literature summary for 6.5.1.4 extracted from
- Cyclases of the 3'-terminal phosphate in RNA: a new family of RNA processing enzymes conserved in Eucarya, Bacteria and Archaea (1998), Acta Biochim. Pol., 45, 895-906.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and overexpressed in Escherichia coli as fusion protein with glutathione S-transferase | Homo sapiens |
| expressed sequence tags encoding cyclase like protein identified | Drosophila melanogaster |
| expressed sequence tags encoding cyclase like protein identified | Mus musculus |
| expressed sequence tags encoding cyclase like protein identified | Saccharomyces cerevisiae |
| expressed sequence tags encoding cyclase like protein identified | Arabidopsis thaliana |
| expressed sequence tags encoding cyclase like protein identified | Dictyostelium discoideum |
| expressed sequence tags encoding cyclase like protein identified | Schizosaccharomyces pombe |
| expressed sequence tags encoding cyclase like protein identified | Caenorhabditis elegans |
| expressed sequence tags encoding cyclase like protein identified | Danio rerio |
| expressed sequence tags encoding cyclase like protein identified | Toxoplasma gondii |
| gene encoding cyclase like protein present | Pseudomonas aeruginosa |
| gene encoding cyclase like protein present | Methanocaldococcus jannaschii |
| gene encoding cyclase like protein present | Aquifex aeolicus |
| gene rtcA encoding cyclase cloned and overexpressed | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
ATP | - |
Homo sapiens | |
| 0.02 | - |
ATP | - |
Escherichia coli | |
| 0.1 | - |
GTP | - |
Escherichia coli | |
| 0.2 | - |
GTP | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Xenopus sp. | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mn2+ | able to replace Mg2+ | Escherichia coli | |
| additional information | no activity with Ca2+, Zn2+, or Cu2+ | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39400 | - |
calculated from cDNA | Homo sapiens |
| 40000 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
| Arabidopsis thaliana | - |
- |
- |
| Caenorhabditis elegans | - |
- |
- |
| Danio rerio | - |
zebra fish | - |
| Dictyostelium discoideum | - |
- |
- |
| Drosophila melanogaster | - |
fruit fly | - |
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
human | - |
| Methanocaldococcus jannaschii | - |
- |
- |
| Mus musculus | - |
mouse | - |
| no activity in Bacillus subtilis | - |
- |
- |
| no activity in Haemophilus influenzae | - |
- |
- |
| no activity in Helicobacter pylori | - |
- |
- |
| no activity in Mycoplasma genitalium | - |
- |
- |
| no activity in Synechocystis sp. | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Saccharomyces cerevisiae | - |
yeast | - |
| Schizosaccharomyces pombe | - |
fission yeast | - |
| Toxoplasma gondii | - |
- |
- |
| Xenopus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
| recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (Ap)npAp | pure oligoribonucleotide | Homo sapiens | AMP + ? | - |
? | |
| ATP + (Up)10pGp | pure oligoribonucleotide | Homo sapiens | AMP + ? | - |
? | |
| ATP + 5S rRNA | modified by ligation of [5'-P]pCp to the 3'-terminus | Escherichia coli | AMP + ? | - |
? | |
| ATP + 5S rRNA-pCp | - |
Homo sapiens | AMP + ? | - |
? | |
| ATP + AAAAUAAAAGCp | pure oligoribonucleotide | Escherichia coli | AMP + ? | - |
? | |
| ATP + AAAAUAAAAGCp | pure oligoribonucleotide | Homo sapiens | AMP + ? | - |
? | |
| ATP + AAAAUAAAAGp | pure oligoribonucleotide | Escherichia coli | AMP + ? | - |
? | |
| ATP + AAAAUAAAAGp | pure oligoribonucleotide | Homo sapiens | AMP + ? | - |
? | |
| ATP + AUGp | pure oligoribonucleotide | Homo sapiens | AMP + ? | - |
? | |
| ATP + CCCCACCCCGp | pure oligoribonucleotide | Escherichia coli | AMP + ? | - |
? | |
| ATP + CCCCACCCCGp | pure oligoribonucleotide | Homo sapiens | AMP + ? | - |
? | |
| ATP + CCCCCACCCCGCp | pure oligoribonucleotide | Homo sapiens | AMP + ? | - |
? | |
| ATP + human U14 snoRNA | modified by ligation of [5'-P]pCp to the 3'-terminus | Escherichia coli | AMP + ? | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Drosophila melanogaster | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Mus musculus | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Escherichia coli | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Homo sapiens | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Saccharomyces cerevisiae | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Arabidopsis thaliana | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Pseudomonas aeruginosa | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Dictyostelium discoideum | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Schizosaccharomyces pombe | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Caenorhabditis elegans | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Xenopus sp. | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Methanocaldococcus jannaschii | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Danio rerio | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Aquifex aeolicus | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | - |
Toxoplasma gondii | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + tobacco mosaic virus RNA | fragments modified by ligation of pGp, pAp, or pCp | Homo sapiens | AMP + ? | - |
? | |
| ATP + tRNA-pNp | - |
Homo sapiens | AMP + ? | - |
? | |
| GTP + RNA 3'-terminal-phosphate | - |
Escherichia coli | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| GTP + RNA 3'-terminal-phosphate | - |
Homo sapiens | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| additional information | nucleoside 3'-monophosphates and nucleoside 5',3'-bisphosphates are no substrates | Escherichia coli | ? | - |
? | |
| additional information | trinucleotides are the shortest oligonucleotides able to act as substrates, ribonucleoside 3'-phosphates and ribonucleoside 5',3'-phosphates are no substrates | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNA 3'-teminal phosphate cyclase | - |
Methanocaldococcus jannaschii |
| RNA 3'-teminal phosphate cyclase | - |
Aquifex aeolicus |
| RNA 3'-terminal phosphate cyclase | - |
Drosophila melanogaster |
| RNA 3'-terminal phosphate cyclase | - |
Mus musculus |
| RNA 3'-terminal phosphate cyclase | - |
Escherichia coli |
| RNA 3'-terminal phosphate cyclase | - |
Homo sapiens |
| RNA 3'-terminal phosphate cyclase | - |
Saccharomyces cerevisiae |
| RNA 3'-terminal phosphate cyclase | - |
Arabidopsis thaliana |
| RNA 3'-terminal phosphate cyclase | - |
Pseudomonas aeruginosa |
| RNA 3'-terminal phosphate cyclase | - |
Dictyostelium discoideum |
| RNA 3'-terminal phosphate cyclase | - |
Schizosaccharomyces pombe |
| RNA 3'-terminal phosphate cyclase | - |
Caenorhabditis elegans |
| RNA 3'-terminal phosphate cyclase | - |
Xenopus sp. |
| RNA 3'-terminal phosphate cyclase | - |
Danio rerio |
| RNA 3'-terminal phosphate cyclase | - |
Toxoplasma gondii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
| ATP | requires ATP as cofactor, other ribonucleoside triphosphates are used very inefficiently | Homo sapiens | |
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