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Literature summary for 6.5.1.3 extracted from

  • Blondal, T.; Hjorleifsdottir, S.H.; Fridjonsson, O.F.; Aevarsson, A.; Skirnisdottir, S.; Hermannsdottir, A.G.; Hreggvidsson, G.O.; Smith, A.V.; Kristjansson, J.K.
    Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 (2003), Nucleic Acids Res., 31, 7247-7254.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
RnlA gene overexpressed in Escherichia coli BL21 Rhodothermus phage RM378

Inhibitors

Inhibitors Comment Organism Structure
NaCl
-
 Rhodothermus phage RM378

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum activity at 5 mM Rhodothermus phage RM378
Mn2+ optimum activity at 1 mM Rhodothermus phage RM378

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51300
-
 calculated from the putative RNA ligase 1 gene Rhodothermus phage RM378

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m Rhodothermus phage RM378
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodothermus phage RM378
-
 thermophilic bacteriophage that infects the thermophilic eubacterium Rhodothermus marinus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Rhodothermus phage RM378

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Rhodothermus phage RM378 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
bacteriophage RNA ligase
-
 Rhodothermus phage RM378
RM378 RNA ligase
-
 Rhodothermus phage RM378
RnlA
-
 Rhodothermus phage RM378
thermostable RNA ligase 1
-
 Rhodothermus phage RM378

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
64
-
-
 Rhodothermus phage RM378

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 90 relatively stable at 60°C with a half-life of 10 h, activity increases after incubation, not stable at temperatures. Enzyme starts to lose activity rapidly, no activity at all after 1 h at 80°C, loses all of its activity after 5 min at 90°C Rhodothermus phage RM378

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 Tris buffer Rhodothermus phage RM378
7
-
 MOPS buffer Rhodothermus phage RM378