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Literature summary for 6.5.1.3 extracted from

  • Atencia, E.A.; Montes, M.; Gunther Sillero, M.A.; Sillero, A.
    Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction (2000), Eur. J. Biochem., 267, 1707-1714.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
5'-adenylated cytidine 5',3'-bisphosphate pH 7.4, 30°C Tequatrovirus T4
4
-
diguanosine tetraphosphate pH 7.4, 30°C Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
SDS-PAGE Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m Tequatrovirus T4
-
AMP + diphosphate + (ribonucleotide)n+m
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylated cytidine 5',3'-bisphosphate + diguanosine tetraphosphate
-
Tequatrovirus T4 Gp4GpCp + pCpGp4GpCp
-
?
ATP + (ribonucleotide)n + (ribonucleotide)m
-
Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
?

Synonyms

Synonyms Comment Organism
T4 RNA ligase
-
Tequatrovirus T4