KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
ATP | - |
Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Tequatrovirus T4 | |
Mg2+ | Km: 0.4 mM, inhibition above 10 mM | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0383 | - |
5'-phosphate terminus rendered resistant to phosphatase | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | converts linear molecules of polyriboadenylate to circular form by the intramolecular covalent linkage of the 5'-phosphate end to the 3'-hydroxyl terminus | Tequatrovirus T4 | AMP + diphosphate + (ribonucleotide)n+m | - |
? | |
ATP + (ribonucleotide)n + (ribonucleotide)m | the best substrate is [5'-P]polyriboadenylate | Tequatrovirus T4 | AMP + diphosphate + (ribonucleotide)n+m | - |
? | |
dATP + (ribonucleotide)n + (ribonucleotide)m | at 20% the activity relative to ATP | Tequatrovirus T4 | dAMP + phosphate + (ribonucleotide)n+m | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.2 | - |
Tequatrovirus T4 |