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Literature summary for 6.5.1.2 extracted from

  • Shrivastava, N.; Nag, J.K.; Pandey, J.; Tripathi, R.P.; Shah, P.; Siddiqi, M.I.; Misra-Bhattacharya, S.
    Homology modeling of NAD+-dependent DNA ligase of the Wolbachia endosymbiont of Brugia malayi and its drug target potential using dispiro-cycloalkanones (2015), Antimicrob. Agents Chemother., 59, 3736-3747 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Wolbachia sp. subsp. Brugia malayi

Inhibitors

Inhibitors Comment Organism Structure
1,6-bis(3,4-dimethoxyphenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
-
 Wolbachia sp. subsp. Brugia malayi
1,6-bis(3,4-dimethoxyphenyl)dispiro[2.1.2(5).3(3)]decan-4-one
-
 Wolbachia sp. subsp. Brugia malayi
1,6-bis(4-bromophenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
-
 Wolbachia sp. subsp. Brugia malayi
1,6-bis(4-fluorophenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
-
 Wolbachia sp. subsp. Brugia malayi
1,6-bis(4-methoxyphenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
-
 Wolbachia sp. subsp. Brugia malayi
1,6-bis(4-methoxyphenyl)dispiro[2.1.2(5).3(3)]decan-4-one
-
 Wolbachia sp. subsp. Brugia malayi
2,7-bis[(3,4,5-trimethoxyphenyl)methyl]cycloheptan-1-one
-
 Wolbachia sp. subsp. Brugia malayi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Wolbachia sp. subsp. Brugia malayi
-
 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
-
 ?
NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Wolbachia sp. subsp. Brugia malayi TRS
-
 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Wolbachia sp. subsp. Brugia malayi Q5GS88
-
-
Wolbachia sp. subsp. Brugia malayi TRS Q5GS88
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Wolbachia sp. subsp. Brugia malayi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Wolbachia sp. subsp. Brugia malayi (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
-
 ?
NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Wolbachia sp. subsp. Brugia malayi TRS (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
-
 ?

Synonyms

Synonyms Comment Organism
LigA
-
 Wolbachia sp. subsp. Brugia malayi
NAD+-dependent DNA ligase
-
 Wolbachia sp. subsp. Brugia malayi

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Wolbachia sp. subsp. Brugia malayi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00102
-
 at pH 8.0 and 20°C Wolbachia sp. subsp. Brugia malayi 2,7-bis[(3,4,5-trimethoxyphenyl)methyl]cycloheptan-1-one
0.0023
-
 at pH 8.0 and 20°C Wolbachia sp. subsp. Brugia malayi 1,6-bis(4-fluorophenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
0.0029
-
 at pH 8.0 and 20°C Wolbachia sp. subsp. Brugia malayi 1,6-bis(4-bromophenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
0.0058
-
 at pH 8.0 and 20°C Wolbachia sp. subsp. Brugia malayi 1,6-bis(3,4-dimethoxyphenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
0.015
-
 at pH 8.0 and 20°C Wolbachia sp. subsp. Brugia malayi 1,6-bis(4-methoxyphenyl)dispiro[2.1.2(5).3(3)]decan-4-one
0.0177
-
 at pH 8.0 and 20°C Wolbachia sp. subsp. Brugia malayi 1,6-bis(4-methoxyphenyl)dispiro[2.1.2(5).2(3)]nonan-4-one
0.354
-
 at pH 8.0 and 20°C Wolbachia sp. subsp. Brugia malayi 1,6-bis(3,4-dimethoxyphenyl)dispiro[2.1.2(5).3(3)]decan-4-one