Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine | IC50 of 0.0001 mg/ml, specific inhibitor of NAD+-dependent DNA ligase, inhibits competitively with respect to NAD+ and specifically inhibits enzyme adenylation, but not DNA adenylation or ligation | Streptococcus pneumoniae | |
methotrexate | IC50 of 0.008 mg/ml | Streptococcus pneumoniae | |
additional information | not inhibited by trimethoprim | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
NAD+ | - |
Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
SDS-PAGE | Streptococcus pneumoniae |
72130 | - |
calculated from amino acid sequence | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose column chromatography and hydroxyapatite column chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Streptococcus pneumoniae | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
LigA | - |
Streptococcus pneumoniae |
NAD+-dependent DNA ligase | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | pH 8.0, 21°C | Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptococcus pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine | - |
Streptococcus pneumoniae |