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Literature summary for 6.5.1.2 extracted from

  • Miesel, L.; Kravec, C.; Xin, A.T.; McMonagle, P.; Ma, S.; Pichardo, J.; Feld, B.; Barrabee, E.; Palermo, R.
    A high-throughput assay for the adenylation reaction of bacterial DNA ligase (2007), Anal. Biochem., 366, 9-17.
    View publication on PubMed

Application

Application Comment Organism
analysis high-throughput assay for the adenylation reaction of bacterial DNA ligase Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-methylthio ADP
-
Escherichia coli
2-methylthio-ATP
-
Escherichia coli
ADP inhibits adenylation reaction Escherichia coli
AMP inhibits adenylation reaction Escherichia coli
ATP inhibits adenylation reaction Escherichia coli
cAMP inhibits adenylation reaction Escherichia coli
Rp-cAMPS
-
Escherichia coli
Sp-cAMPS
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0011
-
37°C, pH 7.4, DNA ligation Escherichia coli 2-methylthio ATP
0.0015
-
37°C, pH 7.4, DNA ligation Escherichia coli Sp-3',5'-cyclic adenosine monophosphothiolate
0.0041
-
37°C, pH 7.4, DNA ligation Escherichia coli 2-methylthio ADP
0.12
-
37°C, pH 7.4, DNA ligation Escherichia coli (Rp)-3',5'-cyclic adenosine monophosphothiolate