Application | Comment | Organism |
---|---|---|
analysis | high-throughput assay for the adenylation reaction of bacterial DNA ligase | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-methylthio ADP | - |
Escherichia coli | |
2-methylthio-ATP | - |
Escherichia coli | |
ADP | inhibits adenylation reaction | Escherichia coli | |
AMP | inhibits adenylation reaction | Escherichia coli | |
ATP | inhibits adenylation reaction | Escherichia coli | |
cAMP | inhibits adenylation reaction | Escherichia coli | |
Rp-cAMPS | - |
Escherichia coli | |
Sp-cAMPS | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0011 | - |
37°C, pH 7.4, DNA ligation | Escherichia coli | 2-methylthio ATP | |
0.0015 | - |
37°C, pH 7.4, DNA ligation | Escherichia coli | Sp-3',5'-cyclic adenosine monophosphothiolate | |
0.0041 | - |
37°C, pH 7.4, DNA ligation | Escherichia coli | 2-methylthio ADP | |
0.12 | - |
37°C, pH 7.4, DNA ligation | Escherichia coli | (Rp)-3',5'-cyclic adenosine monophosphothiolate |