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Literature summary for 6.5.1.2 extracted from

  • Gul, S.; Brown, R.; May, E.; Mazzulla, M.; Smyth, M.G.; Berry, C.; Morby, A.; Powell, D.J.
    Staphylococcus aureus DNA ligase: characterization of its kinetics of catalysis and development of a high-throughput screening compatible chemiluminescent hybridization protection assay (2004), Biochem. J., 383, 551-559.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
Chloroquine diphosphate IC50: 0.0014 mM Staphylococcus aureus
doxorubicin IC50: 0.0033 mM Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000025
-
acridinium-ester labelled DNA pH 7.4, 21°C Staphylococcus aureus
0.00275
-
NAD+ pH 7.4, 21°C Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acridinium-ester labelled DNA
-
Staphylococcus aureus ?
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m acridinium-ester labelled DNA Staphylococcus aureus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Staphylococcus aureus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0014
-
IC50: 0.0014 mM Staphylococcus aureus Chloroquine diphosphate
0.0033
-
IC50: 0.0033 mM Staphylococcus aureus doxorubicin