Application | Comment | Organism |
---|---|---|
medicine | potential target for antibiotics | Thermus filiformis |
Cloned (Comment) | Organism |
---|---|
native and selenomethionine-substituted enzyme | Thermus filiformis |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method | Thermus filiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermus filiformis | |
Zn2+ | 1 mol per mol of enzyme, Cys406, Cys409, Cys422 and Cys427 form a zinc binding motif | Thermus filiformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75940 | - |
calculated from DNA-sequence | Thermus filiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus filiformis | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus filiformis | Q9ZHI0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus filiformis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Thermus filiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus filiformis | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Thermus filiformis | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Thermus filiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Thermus filiformis |