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Literature summary for 6.5.1.2 extracted from

  • Thorbjarnardottir, S.H.; Jonsson, Z.O.; Andresson, O.S.; Kristjansson, J.K.; Eggertsson, G.; Palsdottir, A.
    Cloning sequence analysis of the DNA ligase-encoding gene of Rhodothermus marinus, and overproduction, purification and characterization of two thermophilic DNA ligase (1995), Gene, 161, 1-6.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermus scotoductus
overexpression in Escherichia coli Rhodothermus marinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79487
-
x * 79487, calculation from nucleotide sequence Rhodothermus marinus

Organism

Organism UniProt Comment Textmining
Rhodothermus marinus P49421
-
-
Thermus scotoductus
-
-
-
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus scotoductus
-
Rhodothermus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Thermus scotoductus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m nick-closing activity Rhodothermus marinus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Subunits

Subunits Comment Organism
? x * 79487, calculation from nucleotide sequence Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Rhodothermus marinus
65
-
-
Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 75 active in this range Rhodothermus marinus
15 75 active in this range Thermus scotoductus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
91
-
half-life: 7 min Rhodothermus marinus
91
-
half-life: 26 min Thermus scotoductus