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Literature summary for 6.5.1.1 extracted from

  • Liu, X.; Huang, A.; Luo, D.; Liu, H.; Han, H.; Xu, Y.; Liang, P.
    Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli (2015), Protein Expr. Purif., 109, 79-84 .
    View publication on PubMed
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
an adenylate kinase-T4 DNA ligase fusion protein is expressed in Escherichia coli XL1-Blue cells Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Tequatrovirus T4
-
 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine Tequatrovirus T4
-
 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose and Ni-NTA column chromatography Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Tequatrovirus T4 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine
-
 Tequatrovirus T4 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
T4 DNA ligase
-
 Tequatrovirus T4