Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)Star cells | Psychromonas sp. SP041 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.4, 1.6-1.9 M ammonium sulfate | Psychromonas sp. SP041 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition with ATP concentrations up to 0.5 mM | Psychromonas sp. SP041 | |
NaCl | NaCl concentrations above 100 mM have an inhibitory effect | Psychromonas sp. SP041 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000124 | - |
(nicked double-stranded DNA)n | at 30°C, pH not specified in the publication | Psychromonas sp. SP041 | |
0.0038 | - |
ATP | at 30°C, pH not specified in the publication | Psychromonas sp. SP041 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2 is required for enzyme activity, with an optimal concentration between 1 and 5 mM | Psychromonas sp. SP041 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m | Psychromonas sp. SP041 | - |
(deoxyribonucleotide)n+m + AMP + diphosphate | - |
? | |
ATP + [DNA ligase]-L-lysine | Psychromonas sp. SP041 | - |
[DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychromonas sp. SP041 | A0A0A6YVN6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Psychromonas sp. SP041 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m | - |
Psychromonas sp. SP041 | (deoxyribonucleotide)n+m + AMP + diphosphate | - |
? | |
ATP + (nicked double-stranded DNA)n + (nicked double-stranded DNA)m | - |
Psychromonas sp. SP041 | AMP + diphosphate + (nicked double-stranded DNA)n+m | - |
? | |
ATP + [DNA ligase]-L-lysine | - |
Psychromonas sp. SP041 | [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate | - |
? | |
additional information | in addition to sealing a singly nicked fully matched double-stranded DNA substrate, the enzyme is also able to ligate a cohesive-ended double-stranded break and a single nick with a 1 base pair mismatch at the 3'-OH position of the nick, albeit with reduced efficiency. Ligation of a blunt-end double-stranded break and a 1 nucleotide gapped substrate is also detected when enzyme concentrations and extended incubation times are used; however, no activity is seen with a nonphosphorylated 5'-nicked strand or single-stranded DNA | Psychromonas sp. SP041 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADL | - |
Psychromonas sp. SP041 |
ATP-dependent DNA ligase | - |
Psychromonas sp. SP041 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
(nicked double-stranded DNA)n | at 30°C, pH not specified in the publication | Psychromonas sp. SP041 |