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Literature summary for 6.5.1.1 extracted from
- Improvement of DNA adenylation using T4 DNA ligase with a template strand and a strategically mismatched acceptor strand (2008), Bioorg. Chem., 36, 46-56.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)pLysS cells | Tequatrovirus T4 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Tequatrovirus T4 | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |  |
| additional information | T4 DNA can adenylate the 5'-phosphorylated donor DNA substrate with a DNA template, ATP, and an acceptor strand that has a strategically chosen C-T acceptor-template mismatch directly adjacent to the adenylation site, between 0.5 and 10 mM ATP the adenylation yield is high (about 60%), whereas the yield is lower outside of this range | Tequatrovirus T4 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| T4 DNA ligase | - |
Tequatrovirus T4 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Tequatrovirus T4 | |
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Release 2023.1 (January 2023)
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